通塞脉片对大鼠实验性动脉粥样硬化模型血管内皮细胞的影响  

Effect of Tongsaimai Tablet on experimental atherosclerosis in rats

作　　者：杨雨微[1] 胡晨[1] 卞慧敏[1] 戴晓明[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《中成药》2010年第3期371-374,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(30873401)

摘　　要：目的:研究通塞脉片(当归、党参、黄芪、石斛、川芎、金银花、牛漆、甘草)(TSM)对大鼠实验性动脉粥样硬化内皮细胞的影响。方法:50只♂性wistar大鼠,随机分为空白组、模型组、辛伐他汀组、TSM高剂量和低剂量组,除空白对照组给以普通饲料喂养外,其他组均饲以高脂饲料及维生素D3,建立食饵性动脉粥样硬化模型。12周后检测外周血循环内皮细胞(CEC)、血管紧张素Ⅱ(AngⅡ)、电镜观察动脉血管形态。结果:模型组大鼠CEC明显增加,AngⅡ含量显著升高,TSM能够减少CEC,降低AngⅡ含量。透射电镜观察模型组大鼠主动脉内皮严重受损;TSM组大鼠主动脉内皮细胞扁平形态基本完整,内弹性膜平整厚度均匀,损伤明显减轻。结论:TSM通过减少CEC数量、降低AngⅡ水平,达到保护大鼠实验性动脉粥样硬化模型内皮细胞的作用。AIM：To investigate the effect of TSM tablet on rat＇s atherosclerotic model＇s endothelial cell and explore the mechanism of it.METHODS：Fifty Wistar rats were randomly assigned into five groups：control group,model group,Simvastatin group,TSM high group,TSM low group.All groups were fed with high fat diet and vitamin D3 except for control group to set up atherosclerosis model.After 12 weeks we detected circle endothelial cell,angiotensinⅡ and electron microscopy morphology of arteries.RESULTS：Level of circulating enthelia cell（CEC） and AngⅡin model rats were significantly higher.TSM can reduce level of the CEC and AngⅡ.Model group rat＇s artery endothelial cells were severely damaged under electron microscopy;rat＇s artery endothelial cellsin TSM group were basically intact and its internal elastic membrane was unbroken on it thickness was even,without significant lesion.CONCLUSION：TSM by reducing the number of CEC,the level of AngⅡ used for experimental atherosclerotic endothelial cell inrats has the protective effect.

关 键 词：通塞脉 动脉粥样硬化 外周血循环内皮细胞 血管紧张素Ⅱ 大鼠 

分 类 号：R285.6[医药卫生—中药学]