基于聚类分析对中药活性成分逆转HL60/HTM耐药性评价研究  

Cluster analysis of herb active ingredients in reversal effect on HL60/HT

作　　者：蔡宇[1] 蔡天革[2] 余绍蕾[1] 

机构地区：[1]暨南大学药学院,广东广州510632 [2]辽宁大学生命科学学院,辽宁沈阳110036

出　　处：《中成药》2010年第3期379-382,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(30160098);广东省自然科学基金资助(07117912);广东省医学科学基金资助(33909016)

摘　　要：目的:对具有耐药逆转作用的数种中药成分进行聚类分析。方法:MTT比色法测定补骨脂素、补骨脂甲素、姜黄素、槲皮素、贝母碱、异补骨脂定、补骨脂酚、大黄酸八种中药成分HL60/HT细胞的增殖抑制作用,流式细胞仪测定细胞内化疗药物HT荧光强度及细胞P糖蛋白表达;测定得到的相关数据,通过聚类分析方法进行药物分组。结果:8种药物的逆转倍数在6.2～12.5之间,HL60/HT细胞中HT浓度在9.6～25.6之间,HL60/HT细胞P-gp表达率在29.3～50.1之间。结论:基于4种实验指标可将八种药物按相似性聚类分成3组:补骨脂素、姜黄素、槲皮素3种成分为第一组,补骨脂甲素、贝母碱、异补骨脂定、大黄酸四种成分为第2组,补骨脂酚为第3组。AIM：To do cluster analysis on the herb active ingredients with reversal activity of multi-drug resistance in HL60/HT cells.METHODS：HL60/HT cell was used to check the inhibitory effect of Psoralen,psoralen-A,curcumin,quercetin,psoralidin,isopsoralidin,psoralen phenol,Rhein on cell proliferation by MTT assay.Flow cytometry was used to measure HL fluorescence index and the expression of P-glucose protein.On the basis of the experimental data,herbs were classified as different groups by cluster analysis.RESULTS：Reversion rate,HT concentration and P-gp expression of HL60/HT fell within the range of 6.2 to 12.5,9.6 to 25.6,and 29.3 to 50.1,respectively.CONCLUSION：According to cluster similarity,8 herbal ingredients could be divided into three groups,psoralen,curcumin and quercetin were in the first group.Psoralen-A,psoralidin,isopsoralidin and rhein were in the second group,psoralen phenol was in the third group.

关 键 词：聚类分析 多药耐药 HL60/HT 

分 类 号：R285.6[医药卫生—中药学]