Box-Behnken效应面法优化红旱莲总黄酮提取工艺的研究  

Optimization of extraction of total flavonoids from Hypericum ascyron by Box-Behnken design

作　　者：田宝成[1] 贾昌平[1] 杨军涛[1] 李彦冰[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中成药》2010年第3期389-392,共4页Chinese Traditional Patent Medicine

摘　　要：目的:提出拟合响应曲面的3水平设计(Box-Behnken设计)优化红旱莲中总黄酮回流提取工艺。方法:以总黄酮得率为评价指标,乙醇浓度、提取温度、提取时间及溶剂量为考察因素,采用Box-Behnken效应面法优化回流提取工艺条件,并进行预测分析。结果:试验表明乙醇浓度和提取温度对红旱莲总黄酮提取量具极显著影响,最优提取工艺为乙醇浓度53.2%,提取温度78.7℃,提取时间2.3h,液料比13.3mL/g,二项式拟合复相关系数较高(r=0.9846),回归模型的提取率预测值与测定值偏差率为-4.01%。结论:Box-Behnken效应面法用于优化红旱莲的提取工艺是可行的,建立的数学模型和实验观察数据相符。AIM：To put forward three-level design（Box-Behnken design） for fitting response surfaces to optimize the extraction technology of total flavonoids from Hypericum ascyron L.METHODS：Four factors,including temperature,reflux time,concentration of ethanol,ratio of solvent to raw material were used to examine the yield of total flavonoids.Prediction was carried out through comparing the observed and predicted values.RESULTS：The results suggested that ethanol concentration and extraction temperature were two statistically significant factors.The optimum conditions of extraction process consisted of the ratio（mL：g）of solvent to material（13.3：1）,ethanol concentration（53.2%）,extraction temperature（78.7℃）and extraction time（2.3h）.Regression coefficient of bino-mial fitting complex model was as high as 0.984 6.Bias between observed and predicted values was -4.01%.CONCLUSION：Box-Behnken design is success in optimizing the extaction in close agreement with the predicted values of the mathematic model.

关 键 词：BOX-BEHNKEN设计 红旱莲 总黄酮 

分 类 号：R284.3[医药卫生—中药学]