气相色谱法同时检测莪术油中的莪术醇和β-榄香烯  

Simultaneous determination of curcumol contentin and β-elemene in Zedoary Turmeric Oil by GC

作　　者：刘星[1,2] 程岚[2] 吕国军[1] 赵姗[1] 汪圣华[1,3] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]辽宁中医药大学,辽宁大连116600 [3]中国科学院研究生院,北京100049

出　　处：《中成药》2010年第3期426-429,共4页Chinese Traditional Patent Medicine

基　　金：中国科学院知识创新工程重要方向项目(KJCX2-YW-210-02);中国科学院支持天津滨海新区建设科技行动计划项目(TJZX2-YW-14)

摘　　要：目的:建立了一种同时检测莪术油中莪术醇和β-榄香烯的气相色谱分析方法。方法:以PEG弹性石英毛细管柱(30m×0.32mm)分离,氢火焰离子检测器(FID)检测,氮气为载气,采用程序升温法,即80℃保留7min,以20℃/min的速度升温至140℃保留15min,气化室温度为210℃,检测温度:200℃,柱前压为0.27MPa,进样量为1μL,采用外标法定量。结果:莪术醇在0.2305～3.23mg/mL范围内线性良好,r=0.9993;平均回收率为100.4%,RSD为1.1%,β-榄香烯在0.2375～3.33mg/mL范围内线性良好,r=0.9997;平均回收率为100.2%,RSD为2.2%。结论:本法具有简单、快速、准确、灵敏度高的优点,适用于成分复杂的莪术油体系中莪术醇和β-榄香烯的同时检测。AIM：To develop a new method of gas chromatography for simultaneously determining curcumol contentin and β-elemene in Zedoary Turmeric Oil.METHODS：Separation and quantification were achieved on a quartz capillary column（30 m×0.32 mm） of PEG with a column temperature gradient from 80 ℃（holding for 7 min） to 140 ℃ at a rate of 20 ℃/min,holding for 15 min,taking N2 as loader.The temperature for gasification was 210 ℃;the temperature for detection was 200 ℃;the compressive stress of column was 0.27 MPa.RESULTS：Under the optimal chromatographic conditions,the linear range of curcumol contentin was within 0.2305-3.23 mg/mL and the linear range of the β-elemene was within 0.2375-3.33 mg/mL.The average recovery of curcumol contentin was 100.4% with a relative standard deviation（RSD） of 1.1% and β-elemene was 100.2% with a RSD of 2.2%.CONCLUSION：The results show that it is a simple,rapid,accurate and sensitive method which is suitable for analysis of complex component（curcumol contentin and β-elemene） in Zedoary Turmeric Oil at the same time.

关 键 词：气相色谱法 莪术油 莪术醇 Β-榄香烯 

分 类 号：R927.2[医药卫生—药学]