HPLC测定肉桂、木香中桂皮醛、木香烃内酯和去氢木香内酯  

Assay of cinnamaldehyde,costunolide and dehydrocostuslactone in Cinnamomum cassia and Aucklandia lapp by HPLC

作　　者：贾晓斌[1,2] 王丽静[1,2] 陈彦[2] 宋师花[1,2] 王绪颖[1,2] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏省中医药研究院中药新型给药系统重点实验室/国家中医药管理局中药口服释药系统重点研究室,江苏南京210028

出　　处：《中成药》2010年第3期459-462,共4页Chinese Traditional Patent Medicine

基　　金：江苏省公益专项(BM2007701);江苏省中医药领军人才项目(2006;2009)

摘　　要：目的:建立以高效液相色谱法测定肉桂、木香CO2超临界萃取物中桂皮醛、木香烃内酯和去氢木香内酯含量的方法。方法:色谱柱为Agilent HC-C18(150mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),检测波长210nm,流速1.0mL/min,柱温30℃。结果:桂皮醛进样量在148.5～1732.5ng与峰面积有良好的线性关系,平均回收率为99.65%,RSD为0.72%;木香烃内酯进样量在69.42～809.9ng与峰面积有良好的线性关系,平均回收率为99.57%,RSD为1.28%;去氢木香内酯进样量在70.32～820.4ng与峰面积有良好的线性关系,平均回收率为98.90%,RSD为0.81%。结论:本方法简便、准确、重复性好,可用于肉桂、木香CO2超临界萃取物的质量控制。AIM：To establish an HPLC method for determining cinnamaldehyde,costunolide and dehydrocos-tuslactone in the supercritical carbon dioxide extraction of Cinnamomum cassia and Aucklandia lapp.METHODS：The assay was performed on an Agilent HC-C18（150 mm×4.6 mm,5 μm） column by UV detector at the wave-length of 210 nm with acetonitrile-water（gradient elutio） as the mobile phase at the flow rate of 1.0 mL/min,and the column temperature was 30℃.RESULTS：There were good relationships between peak area and sample size of cinnamaldehyde in the range of 148.5-1732.5 ng,between peak area and sample size of costunolide in the range of 69.42-809.9 ng,and between peak area and sample size of dehydrocostuslactone in the range of 70.32 to 820.4 ng.Average recoveries of them were in turn 99.65%（RSD 0.72%） -99.57%（RSD 1.28%）,and 98.90%（RSD 0.81%）,respectively.CONCLUSION：The present method is convenient,sensitive and accurate with good reproducibility and can be used for the quality control of the supercritical CO2 extract of Cinnamomum cassia and Aucklandia lapp.

关 键 词：高效液相色谱法 桂皮醛 木香烃内酯 去氢木香内酯 含量测定 

分 类 号：R284.1[医药卫生—中药学]