尾静脉注射STZ制备1型糖尿病大鼠模型实验的技巧  

Empirical Skills of Establishing Type 1 Diabetie Model Rat by Injection Through Vena Caudalis Streptozotocin

作　　者：王继红[1] 刘学政[2] 杨雪佳[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁医学院,辽宁锦州121000

出　　处：《辽宁医学院学报》2010年第1期7-9,共3页Journal of Liaoning Medical University (LNMU) Bimonthly

摘　　要：目的探讨STZ尾静脉不同给药方法和补造模时不同给药剂量致大鼠糖尿病成模状况的差异。方法本实验通过尾静脉不同的给药方法(非盐水导入组、盐水导入组)以建立大鼠糖尿病模型。以及未成模的大鼠给予不同剂量的STZ补造模(全量组、2/3全量组)。观察给药后大鼠成模情况、一般状况及检测各组成模后3 d、4 w、8 w血糖值的变化。结果盐水导入方法可以大幅度降低STZ尾静脉注射造模时的死亡率(6.52%),并提高成模率(73.9%)。补造模时2/3剂量组死亡率降低(6.67%,P<0.05)且成模后血糖各组间,各时间点无差异(P>0.05)。结论盐水导入的尾静脉给药方法是注射链脲佐菌素致大鼠糖尿病模型的最佳途径,补造模时以2/3剂量为宜。Objective To explore the impact on induction of diabetes in rat models with different STZ administration and dosage. Methods SD rats received caudal vein injection of STZ to induce diabetes, with or without saline preceding the STZ injection. The impact of different additional amounts of STZ on the induction of diabetic model was also observed. The percentage of diabetic models, general condition and the blood glucose were determined on 3d, 4w and 8w after STZ injection. Results The method of saline usage significantly decreased the mortality （6. 52% ） and increased the percentage of diabetic models （73.9%）. The additional amount of STZ had its lowest mortality （6. 67% ） when 2/3 routine amount was used, meanwhile the differences of blood glucose remained no significant compared with the control group. Conclusions The administration of saline preceding STZ injection is better than STZ injection alone, and if necessary, a 2/3 additional amount of STZ should be used.

关 键 词：链脲佐菌素 盐水导入法 糖尿病模型 大鼠 

分 类 号：R335[医药卫生—人体生理学]