Triton X-100共振能量转移荧光猝灭法测定尿中α-萘酚  

Determination of α-naphthol in urine with Triton X-100 using resonance energy transfer fluorescence quenching

作　　者：王英 王永生[2] 

机构地区：[1]衡阳市中心血站,湖南衡阳421001 [2]南华大学公共卫生学院,湖南衡阳421001

出　　处：《应用化工》2010年第3期453-455,共3页Applied Chemical Industry

基　　金：湖南省自然科学基金课题(03JJY3030);湖南省卫生厅科研基金课题(B2003-077)

摘　　要：研究了α-萘酚与TritonX．100之间的共振能量转移的最佳条件，建立了TritonX-100共振能量转移荧光猝灭法测定α-萘酚的新方法。在pH7．5的Tris-HCl缓冲溶液中，在300．8nm，TritonX-100的荧光猝灭强度与α-萘酚浓度成正比，工作曲线的线性范围为0．36～1．80μg/L，方法的检出限为0．12ng／mL，RSD为0．7％-1．1％，平均加标回收率为100．9％（n=6）。该方法已用于尿样测定，结果满意。The optimum conditions for the resonance energy transfer between α-naphthol and Triton X-100 were studied, and a novel method for the determination of α-naphthol has been developod based on the resonance energy transfer fluorescence quenching effect of Triton X-100, the fluorescence quenching intensity of Triton X-100 at 300.8 nm was proportional to the concentration of α-naphthol. The linear range of the calibration curve was 0.36 - 1.80 μg/L in the pH 7.5 Tris-HCl buffer solution with the detection limits of 0.12 ng/mL. The relative standard deviation was 0.7% - 1.1% and the average recovery was 100.9%. The proposed method has succeeded in analyzing the spiked urine sample with satisfactory results.

关 键 词：Α-萘酚 TRITON X-100 荧光猝灭 共振能量转移 

分 类 号：O625.15[理学—有机化学]