氟中毒大鼠血清中NO和MDA及GSH-Px的研究  

CHANGES OF NO AND MDA AND GSH-Px IN FLUORIDE-INTOXICATED RATS

作　　者：刘连[1] 徐园园[2] 许洁[2] 李勇[2] 刘振环[2] 黄厚今[2] 

机构地区：[1]遵义医学院组织学与胚胎学教研室,遵义563003 [2]遵义医学院预防医学教研室

出　　处：《现代预防医学》2010年第6期1108-1109,共2页Modern Preventive Medicine

基　　金：贵州省自然科学基金(黔科合J字[2008]2192号);贵州省省长基金(c-240)

摘　　要：[目的]研究不同浓度氟对大鼠血清自由基和氧化应激的影响。[方法]在饮水中加入含NaF(分析纯)10,20,40mg/kg的去离子水灌胃喂饲大鼠3月后,测定尿氟、血清氟含量、血液中的钙浓度。血清中的一氧化氮(NO)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)的含量。[结果]随染氟剂量的增加,3月后各剂量组大鼠尿氟浓度相应增加,存在剂量-效应关系(R=0.914,P﹤0.01),与对照组相比有统计学差异(P﹤0.01);各剂量组血氟浓度相应增加,存在剂量-效应关系(R=0.930,P﹤0.01),与对照组相比有统计学差异(P﹤0.05);各剂量组大鼠血钙浓度相应下降,存在剂量-效应关系(R=-0.899,P﹤0.01),与对照组相比有统计学差异(P﹤0.01);各剂量组NO的含量相应增加,存在剂量-效应关系(R=0.951,P﹤0.01),与对照组相比有统计学差异(P﹤0.01);各剂量组MDA含量升高,存在剂量-效应关系(R=0.923,P﹤0.01),与对照组相比有统计学差异(P﹤0.05);各剂量组GSH-Px的活性下降,存在剂量-效应关系(R=-0.966,P﹤0.01),与对照组相比有统计学差异(P﹤0.05),特别是高氟剂量组NO及MDA的含量最高而GSH-Px的活性最低。[结论]慢性氟中毒可导致大鼠处于氧化应激状态。[Objective] To study the effect of different dosages of fluoride in serum on the free radicals and oxidative stress of rats. [Methods] Drinking water with various concentrations of sodium fluoride was given to rats for 3 month. Then, the contents of the fluoride in the urine and serum, concentration of calcium in blood, content of nitnic oxide （NO） and maleic dialdehyde （MDA） and activities of Glutathion Peroxidase （GSH-Px） in serum were measured. [Results] With the increase of content of fluorin, the content of fluorin in urine was increased in all dosage groups after three months. Dose-response effect was existed （R=0.914, P﹤0.01）, and it has significant difference when compare with the controls （P﹤0.01）; The content of NO and MDA increased in all dosage groups, and dose--response effect was existed as well （NO： R= 0.951, P﹤ 0.01; MDA： R=0.923, P﹤0.01）, also, it has significant difference when compare with the controls （NO： P﹤0.01; MDA： P﹤0.05）. The activities of GSH-Px decreased, a dose-response effect presented, and there was significant difference compared with the control group （P﹤0.05）. Especially the contents of NO and MDA was the highest while the activities of GSH-Px was the lowest in the maximal fluoride group. [Conclusion] Chronic fluorosis may induce oxidative stress to rats.

关 键 词：氟中毒 一氧化氮 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R595[医药卫生—内科学]