结肠炎散质量标准研究  

Study on the quality standards of Jiechangyan San

作　　者：李玉华 何继祥 程静 

机构地区：[1]云南曲靖市妇幼医院,云南曲靖655000 [2]云南曲靖市食品药品检验所,云南曲靖655000

出　　处：《中国民族民间医药》2010年第5期42-43,45,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立肠炎散的质量标准。方法:以盐酸小檗碱为对照品,正丁醇-冰醋酸-水(5∶1∶1)为展开剂对其中黄连药材进行薄层色谱鉴别;以防风为对照药材,三氯甲烷-甲醇(4∶1)为展开剂对其中防风药材进行薄层色谱鉴别;以延胡为对照药材、延胡索乙素为对照品,正己烷-氯仿-甲醇(7.5∶4∶1)为展开剂对其中延胡药材进行薄层色谱鉴别;采用反相高效液相色谱法测定主药黄连中盐酸小檗碱含量,色谱条件:Hypersil C18柱(4.6mm×50mm,5μm)为色谱柱,乙腈-0.03mol/L磷酸二氢钾溶液(55∶45)为流动相;检测波长:265nm。结果:薄层色谱斑点清晰,重现性好,阴性对照无干扰;HPLC理论板数以盐酸小檗碱计算为4100,线性范围0.021-0.1μg,r=0.999 6,平均回收率为100.21%(RSD为0.49%)。结论:本法简便准确,重现性好,可用于该制剂的质量控制。Objective： To establish the quality standards of Jiechangyan Sano Methods： Use berberinc hydrochloride as reference and n - butanol - acetic acid - - waters （5 ： 1 ： 1 ） as developer to indicate rhizoma coptidis by TLC ; Use radix saposhnikoviae as reference and chloroform- -methanol （4：1 ） as developer to indicate radix saposhnikoviae by TLC ; Use rhizoma corydalis and tetrahydropalmatJne as reference and n -hexane -chloroform - - methanol （7. 5： 4：1 ） as developer to indicate rhizoma corydalis by TLC ; Using HPLC method with Hypersil ODS2 C18 column , the mobile phase was a mixture of acetonitrile -0. 03mol· L^-1 potassvium Dihydrogen Phosphate acid （55：45）, the column temperature was room temperature, with the detection wavelength at265nm. Resuits： the linear range was 0. 021 - 0. 1μg, r = 0. 999 6, the average recovery was 100. 21%, RSD = 0. 49% . Conclusion： the methods was simple and accurate with good reproducibility and can be applied to the quality control of Jiechangyan San.

关 键 词：肠炎散 盐酸小檗碱 防风 延胡 延胡索乙素 TLC HPLC 

分 类 号：R284.1[医药卫生—中药学]