不同发育时期小鼠胸腺形态学观察  

Observation of the Morphology on Different Growth Period Mouse Thymus

作　　者：林卡莉[1] 林卡勤 钟乐 陈方[3] 陈卫琴[3] 

机构地区：[1]赣南医学院组织胚胎教研室,江西赣州341000 [2]全南县人民医院,江西全南341800 [3]赣南医学院

出　　处：《赣南医学院学报》2010年第1期21-23,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：目的:探讨不同发育时期小鼠胸腺形态结构变化。方法:分别观察出生后20～25d、9～10周龄及12月龄小鼠的胸腺结构;通过S100免疫组化标记,计算S100+胸腺树突状细胞(TDCs)在参照物(胸腺)中所占面积百分比;计算TDCs密度。结果:随着年龄的增长,小鼠胸腺皮质变薄,皮髓质界限清楚。胸腺髓质由散在斑片状连成片。12月龄小鼠胸腺实质部分明显减少,而被充满脂肪细胞的间质所取代。9～10周龄小鼠TDCs所占面积比和TDCs密度均比20～25d龄的增加,然而12月龄者则明显减少(P<0.01)。12月龄小鼠胸腺皮髓质交界处有较多S100+巨噬细胞。结论:老年鼠TDCs抗原提呈作用的减弱与TDCs数量的减少有密切关系。Objective： to explore the morphologie change on different growth period mouse thymus. Methods： Thymus morphology and thymus dendritic cell （TDCs）immunostaining with DC marker（ S100 ） were observed and analyzed with stereologic method. Results： Following aging, thymie cortex thinningz and limit between cortex and medulla were more distinct, and glandular substance of 12-months mouse decreased obviously, and substituted with interstitial substance to be full of adipocyte. S100^ ＋ TDCs area and TDCs density in 9 - 10-weeks mouse were significantly higher than in 20-25-days mouse , but in 12-months mouse decreased obviously （P 〈 0.01 ） . simultaneously, there were more S100^＋ macrophage on juncture between cortex and medulla in 12-months mouse thymus. Condusion： Lower angtigen presentating effect of TDCs in gerontism mouse were related to lower quantity of TDCs.

关 键 词：小鼠 胸腺 发育 形态学 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]