阳离子型Gemini表面活性剂共振光散射法测定DNA及相互作用机理的研究  

Detection of DNA with a cationic gemini surfactant by resonance light scattering technique and investigation of the interaction mechanism

作　　者：盛立娇[1] 祁红林[1] 宋功武[1] 周建刚[1] 

机构地区：[1]湖北大学有机功能分子合成与应用教育部重点实验室,武汉430062

出　　处：《胶体与聚合物》2010年第1期32-36,共5页Chinese Journal of Colloid & Polymer

基　　金：湖北省自然科学基金资助项目(2008CDB017);有机功能分子合成与应用教育部重点实验室资助项目(2006-KL-006)

摘　　要：在酸性介质(pH=3.51)中,脱氧核糖核酸(DNA)能显著地增强阳离子型Gemini表面活性剂(G14-4-14)的共振光散射信号,据此提出了G14-4-14共振光散射法测定DNA的新方法,并对介质酸度、离子强度、G14-4-14浓度及共存物质干扰等实验条件进行了优化。在最佳条件下绘制了工作曲线,该方法线性范围为0～2760μg.L-1,检出限为7.79μg.L-1,用于合成样品的测定,结果令人满意。以芘(pyrene)为荧光探针,应用稳态荧光法探讨了G14-4-14与DNA的相互作用,并运用红外光谱及共振光散射光谱初步探讨了G14-4-14与DNA相互作用的机理。Based on the enhanced resonance light scattering （RLS） signals of a cationic Gemini surfactant G14-4-14 by DNA, a new detection assay is proposed at pH 3.51. The effects of pH, ionic strength, G14-4-14 concentration and coexisting substances on the RLS enhancement are discussed, respectively. The intensity of RLS signal is proportional to the concentration of DNA in the range from 0 to 2760μg·L^-1 with the determination limit（3σ） of7.791μg·L^-1 . This novel method has been applied to the assay for four synthetic samples with satisfactory results. The interaction is studied by applying pyrene as fluorescence reagent. Infra-red and RLS spectra are used to study the interaction mechanism.

关 键 词：GEMINI表面活性剂 脱氧核糖核酸 共振光散射技术 红外光谱 芘探针 

分 类 号：O657.3[理学—分析化学]