陇东野生紫花苜蓿的同工酶分析  

Isozymes analysis of Longdong wild alfalfa

作　　者：陈立强[1] 师尚礼[1] 满元荣[1] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室中美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2010年第1期24-27,32,共5页Grassland and Turf

基　　金："十一五"国家科技支撑计划(2006BAD01A19;2006BAD04A04-03;2007BAD52B06);农业部公益性行业(农业)科研专项(nyhyzx07-022);国家牧草产业技术体系专项资助

摘　　要：利用聚丙烯酰胺垂直板凝胶电泳技术对陇东野生紫花苜蓿及其他37个紫花苜蓿品种的过氧化物酶、过氧化氢酶和酯酶进行了分析。通过对供试材料间相似系数的计算、聚类分析和主成分分析，初步探讨了38个紫花苜蓿材料间的亲缘关系。结果表明，供试材料共表现出了24条酶带，其中有5条是陇东野生紫花苜蓿的特征酶带。38个紫花苜蓿材料的相似系数介于0．583～1．000，陇东野生紫花苜蓿与其他37个供试材料间的相似系数介于0．583～0．833，远远低于其他供试品种间的相似系数（0．625～1．000），聚类分析表明，供试材料在相似系数为0．702处可聚为2类，其中陇东野生紫花苜蓿单独聚为一类，其结果与主成分分析一致，由此可确定陇东野生紫花苜蓿与其他供试材料间的亲缘关系较远，为一相对独立的紫花苜蓿种质资源，以期通过育种途径扩大品种的遗传基础。Peroxidase, catalase and esterase of Longdong wild alfalfa and other 37 alfalfa （Medicago sativa） cultivars were analyzed using polyacrylamide gel electrophoresis. The results showed that total 24 isozyme bands presented at the tested materials, five of which were only presented at zymogram of Longdong wild alfalfa. Simi- larities varied from 0. 583 to 1. ~300 among 38 varieties, and the similarity between Longdong wild alfalfa and other cultivars varied from 0. 583 to 0. 833 ,which were much lower than other cultivars（0. 625-1. 000）. Cluster analysis indicated that 38 alfalfa varieties can be divided into two major groups at the similarity value of 0. 702, Longdong wild alfalfa was clustered in an independent group,this result was consistent with the results based on principal component analysis. Longdong wild alfalfa can be regarded as distinct alfalfa germplasm,with the potential to broaden the genetic basis of cultivars developed by breeding programs.

关 键 词：陇东野生紫花苜蓿 同工酶 亲缘关系 聚类分析 主成分分析 

分 类 号：S541.035[农业科学—作物学]