利用全测序基因组数据对被子植物纤维素合成酶基因的进化分析  

Genome-Wide Comparative Analysis of Angiosperm Cellulose Synthase Gene

作　　者：王颖[1] 杨鹏[1] 于娅[1] 赵冰[1] 郭仰东[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《植物遗传资源学报》2010年第2期179-185,共7页Journal of Plant Genetic Resources

基　　金：国家重点基础研究发展计划(2009CB119000);国家科技支撑计划(2007BAD57B04);教育部新世纪优秀人才支持计划(NCET-05-0133)

摘　　要：利用已经分离的植物纤维素合成酶基因的Cellulose_synt结构域为检索序列,从NCBI和其他数据库中调取已完成测序的物种的纤维素合成酶的氨基酸序列,共涉及10个物种的171个基因,基于以上氨基酸序列,应用MEGA4.0生成系统进化树。结果表明:CesA基因和Csl基因直向的相似度远大于平行的相似度,且它们的分化可能在单子叶和真双子叶植物分化之前,单子叶和真双子叶植物的最近共同祖先中至少存在7个CesA基因,综合已知的模式植物CesA基因的功能(初生壁或次生壁形成特异性),可为推测其他物种中该基因的功能提供帮助。The cellulose synthase genes were searched and analyzed by using the Cellulose_synt domain sequences identified from the CesA genes in plants as queries in NCBI and other genome databases. Phylogenetic trees of cellulose synthase gene from 171 sequences of CesA in 10 species including eudicot and monocot published on the GenBank were generated by MEGA4.0. The results showed that these cluster in groups wherein orthologs were often more similar than paralogs,indicating that different subclasses evolved prior to the divergence of the monocot and eudicot lineages. These CesA genes in eudicot and monocot were originated from seven genes. Based on the information of biosynthesis of primary or secondary wall by CesA gene regulation in model plant,the function of some unknown genes could be speculated from the trees.

关 键 词：纤维素合成酶基因 被子植物 基因组 系统发育分析 

分 类 号：Q943[生物学—植物学]