SSR标记分析种子老化及繁殖世代对大豆种质遗传完整性的影响  

Effect of Seed Aging and Regeneration on Genetic Integrity in Soybean by SSR Markers

作　　者：王栋[1] 卢新雄[1] 张志娥[1] 陈晓玲[1] 辛霞[1] 辛萍萍[1] 耿立格[2] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]河北省农林科学院粮油作物研究所,石家庄050031

出　　处：《植物遗传资源学报》2010年第2期192-199,共8页Journal of Plant Genetic Resources

基　　金：国家科技支撑项目(2006BAD13B10);农作物种质资源保护利用专项(2130135)

摘　　要：选取大豆品种中黄18为试验材料,利用60对SSR核心引物对经过不同老化时间处理的群体及其繁殖后代群体进行遗传完整性分析。经过老化处理的群体及其繁殖子代群体与未经老化处理的对照群体相比,等位基因频率、有效等位基因数没有显著差异,结果表明经过老化处理种子及其繁殖子代群体的等位基因频率变化不大。未经老化处理、发芽率为98%的对照群体(G_0-1)与其繁殖一代、繁殖二代群体相比,等位变异数、遗传多样性指数、香农指数和稀有等位基因数没有显著差异,且遗传一致度相对较高;而经过老化处理、发芽率低于85%的群体(G_0-3和G_0-4)及其后代繁殖群体与对照群体相比,等位变异数、遗传多样性指数、香农指数和稀有等位基因数显著或极显著降低,且遗传一致度相对较低。因此,种子老化较繁殖世代对大豆种质群体的遗传结构影响更大。In this study soybean cultivar Zhonghuang 18 was used as materials, and the genetic integrity of population with different germination percentage and their descendant population was investigated by 60 core SSR primers. The results showed that there was no significant difference in the allelic frequencies and the effective number of alleles of the aged population and their descendant population compared with the control population. It indicated that the allelic frequencies of the aged population and their descendant population changed slightly. There was no significant difference in the number of alleles, the index of genetic diversity, Shannon＇s information index and the number of rare alleles between the control population with the germination percentage of 98% and its first and second descendant population, and the value of genetic identity was relatively high. The number of alleles, the index of genetic diversity, Shannon＇s information index and the number of rare alleles of the aged population （G0- 3 and G0- 4） with the germination percentage below 85% and their first and second descendant population were significantly reduced compared with the control, and the value of genetic identity was relatively high. Therefore, seed aging maybe had a greater impact on the genetic composition of the soybean population than regeneration times.

关 键 词：大豆 种子老化 繁殖世代 SSR 遗传完整性 种质保存 

分 类 号：S565.1[农业科学—作物学]