大豆品种早熟18抗疫霉根腐病基因的SSR分子标记  

Molecular Mapping of Phytophthora Resistance Gene in Soybean Cultivar Zaoshu 18

作　　者：姚海燕[1,2] 王晓鸣[1] 武小菲[1] 肖炎农[2] 朱振东[1] 

机构地区：[1]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081 [2]华中农业大学植物科学技术学院,武汉430070

出　　处：《植物遗传资源学报》2010年第2期213-217,共5页Journal of Plant Genetic Resources

基　　金：公益性行业(农业)科研专项(3-20)"十一五"国家科技支撑计划(2006BAD08A08);中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项(082060302-06);作物种质资源保护子项目(NB06-070401(23-27)-18)

摘　　要：大豆品种早熟18是抗疫霉根腐病的有效抗源。本研究鉴定和分子标记早熟18的抗疫霉根腐病基因,以期为该品种的有效利用及分子辅助育种奠定基础。以感病大豆品种Williams与早熟18杂交建立分离群体。抗性遗传分析表明,早熟18对大豆疫霉菌抗性由1个显性单基因控制,该基因被定名为RpsZS18。SSR标记连锁分析表明,RpsZS18位于大豆分子遗传连锁群D1b上的SSR标记Sat_069和Sat_183之间,与这两个标记的遗传距离分别为10.0 cM和8.3 cM。RpsZS18是D1b连锁群上鉴定的第一个抗疫霉根腐病基因。Soybean ev. Zaoshu 18 had been identified as an effective source resistant to Phytophthora root rot of soybean. The objectives of this study were to identify and map the Phytophthora resistance gene in Zaoshu 18 in order to facilitate its utilization and marker-assisted breeding. Zaoshu 18 was crossed with susceptible cultivar Williams to develop mapping populations. Genetic analysis of F2and F2：3 progenies indicated that resistance to Phytophthora sojae in Zaoshu 18 was controlled by a dominant single gene. The resistance gene was temporarily designated as RpsZS18. Based on linkage analysis of SSR markers,RpsZS18 was located on soybean molecular linkage group （MLG） D1 b between SSR markers Sat_069 and Sat_183 with genetic distances 10.0cM and 8.3cM, respectively. It was the first locus for Phytophthora resistance that had been identified on MLG D1b.

关 键 词：大豆 早熟18 疫霉根腐病 抗病基因 SSR标记 

分 类 号：S565.1[农业科学—作物学]