高效液相色谱法测定米诺地尔搽剂中米诺地尔的含量  

The Content of Minoxidil about Minoxidil Liniment by High Performance Liquid Chromatography

作　　者：范玉峰[1] 胡守莲 

机构地区：[1]河南省信阳职业技术学院附属医院,464000 [2]河南省信阳市食品药品检验所,464000

出　　处：《中国医药指南》2010年第8期42-43,共2页Guide of China Medicine

摘　　要：目的建立测定米诺地尔搽剂中米诺地尔含量的高效液相色谱(HPLC)检验方法。方法采用Nova-PaKC18色谱柱(150mm×4.6mm,5μm),流动相为甲醇-水(70∶30),流速为1.0mL/min,检测波长为285nm。结果米诺地尔在2.5～20mg/L浓度范围内含量与峰面积比呈现良好的线性关系(r=0.9999),平均回收率为99.5%,RSD为1.85%,最低检出浓度为0.5mg/L。结论本法能准确检测米诺地尔搽剂中米诺地尔的含量,因此可作为米诺地尔搽剂质量控制的有效测量方法。Objective A HPLC method was established for the determination of minoxidil in compound minoxidil liniment. Method A Nova- PaKC^18（150mm×4.6mm,5μm） column was used with the mobile phase of methanolowater（70 ： 30）at the detection wavelength of 285nm.The flow rate was 1.0mL/min. Result The linear range was 2.5-20mg/L（r=0.9999）.The average recovery and RSD were 99.5% and 1.85% respectively. The minimum detectable concentration was 0.5mg/L. Conclusion The method could be used for the determination of minoxidil in compound minoxidil liniment. Therefore it could be used as a effective quality control method for minoxidil liniment.

关 键 词：米诺地尔搽剂 高效液相色谱法 米诺地尔 

分 类 号：R927.2[医药卫生—药学]