TNF-α体外诱导rMSC向神经细胞分化的研究  

Differentiation of rat mesenchymal stem cells into neuron-like cells induced by TNF-α in vitro

作　　者：娄远蕾[1] 邓志锋[2] 赖贤良[2] 谢安[1] 冯年花[1] 郭菲[1] 汪泱[1] 

机构地区：[1]南昌大学泌尿外科研究所 [2]南昌大学第二附属医院神经外科,江西南昌330006

出　　处：《实验与检验医学》2010年第1期25-26,50,共3页Experimental and Laboratory Medicine

基　　金：江西省卫生厅资助项目(20081056)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)诱导大鼠骨髓间充质干细胞向神经细胞分化的作用。方法采用密度梯度离心法分离rMSCs,取培养至3～5代的rMSCs以含TNF-α 5ng/ml的培养基培养,镜下观察细胞诱导后形态的变化,采用免疫荧光细胞化学染色法检测神经前体细胞标志物巢蛋白nestin和神经元特异性烯醇化酶在细胞中的表达强度和分布。结果rMSCs经TNF-α诱导6h后基本表现为典型神经样细胞形态,nestin和NSE呈阳性表达。结论TNF-α具有体外诱导骨髓间充质干细胞向神经样细胞分化的作用。Objective To investigate whether TNF-α can induce neuron-like cells formation from rat marrow stromal cells （rMSCs） in vitro. Methods rMSCs were isolated from bone marrow by density gradient cen- trifugation with Fieoll-Paque and ceils between passeges 3-5 were induced by TNF-α at 5ng/ml. The cellular morphological changes of induced rMSCs were observed by microscope and we monitored expression of nestin and neuronal specific enolase（NSE） in rMSC differention by indirect immuno-fluorescent cytochemistry staining. Results After treated with TNF-α in 6 hours, rMSCs showed obviously morphological changes and expressed the protein for nestin and NSE. Conclusion TNF-α can induce rMSCs into neuron-like cells.

关 键 词：肿瘤坏死因子-α(TNF—α) 骨髓间充质干细胞(MSCs) 神经细胞 分化 

分 类 号：R446.63[医药卫生—诊断学]