Determination of Chlorobenzene by Hydroxypropyl-β- Cyclodextrin Sensitized Fluorescence Quenching Method with Neutral Red as a Fluorescence Probe  

作　　者：G.Z. Zou M. Qian X.L. Zhang 

机构地区：[1]School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China

出　　处：《Journal of Environmental Science and Engineering》2010年第2期9-13,共5页环境科学与工程（英文版）

基　　金：This project was supported by the National Basic Research Program of China （No. 2007CB936602） and the Natural Science Foundation of Shandong Province in China （No. Y2008B20）.

摘　　要：The fluorescence quenching of inclusion complex of neutral red （NR） and hydroxypropyl-β-cyclodextrin （HP-β-CD） carried by chlorobenzene was investigated. The fluorescence intensity of NR increased due to the formed inclusion complex of HP-β-CD and NR. But the fluorescence intensity of NR-HP-β-CD diminished when chlorobenzene was added, and there was a linear relationship between the fluorescence quenching value of the system （△IF = IF, NR-HP-β-CD - IF, CB-NB-NR-HP-β-CD） and the concentration of chlorobenzene. Based on this, a novel fluorescence quenching method for the determination of chlorobenzene with NR as a fluorescence probe has been developed. Under the optimal conditions, the linear range of calibration curve for the determination of chlorobenzene was 5.0 × 10^-8 - 8.0 × 10^-6 mol/L and the detection limit was 1.0 × 10^-8 mol/L. It has been applied to determination ofchlorobenzene in synthetic waste water samples with satisfactory results.

关 键 词：CHLOROBENZENE CYCLODEXTRIN neutral red. fluorescence quenching method 

分 类 号：O614.121[理学—无机化学] TQ460.4[理学—化学]