葡萄籽原花青素对复发性结肠炎大鼠血清抗氧化能力及NO含量的影响  

Effects of proanthocyanidins from grape seeds on the antioxidation in the resistance to oxidation and NO comtents

作　　者：王艳红[1,2] 杨孝来[3] 王莉[1,2] 崔明霞[1,2] 翟晶[3] 吴勇杰[1,2] 

机构地区：[1]兰州大学基础医学院药理学研究所 [2]甘肃省新药临床前研究重点实验室 [3]甘肃省人民医院药剂科

出　　处：《中国临床药理学与治疗学》2010年第1期47-52,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：甘肃省自然科学基金资助项目(3ZS061-A25-083);国家自然科学基金资助项目(30672490)

摘　　要：目的:观察葡萄籽原花青素(GSPE)对复发性结肠炎大鼠血清抗氧化能力及NO含量的影响,初步探讨葡萄籽原花青素治疗复发性结肠炎的作用机制。方法:直肠给予雄性Wistar大鼠80mg/kg2,4,6-三硝基苯磺酸(TNBS)/50%乙醇溶液复制结肠炎模型,在第16天时,用30mg/kgTNBS/50%乙醇溶液诱导结肠炎复发的模型。大鼠第二次致炎24h后,分别应用GSPE低、中、高剂量(100、200、400mg/kg)灌胃对其进行治疗,并以柳氮磺吡啶(SASP,500mg/kg)作为阳性对照。连续给药7d后处死所有大鼠,取结肠标本评价结肠湿重指数,生化法检测血清中髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和一氧化氮合酶(iNOS)活力及丙二醛(MDA)、谷胱甘肽(GSH)和NO含量。结果:与模型对照组比较,GSPE各剂量组大鼠体重下降程度较轻(P<0.05或P<0.01),结肠湿重指数明显降低(P<0.05或P<0.01);大鼠血清中MPO和iNOS活力及MDA和NO含量均明显降低(P<0.05或P<0.01);大鼠血清中SOD和GSH-Px活力及GSH含量明显升高(P<0.05或P<0.01)。结论:GSPE可能通过提高复发性结肠炎大鼠血清抗氧化能力,抑制NO生成,来减轻复发性结肠炎炎症反应。AIM： To investigate the antioxidation of proanthocyanidins from grape seeds （GSPE） on 2, 4, 6-trinitrobenzene sulfonic acid （TNBS） twice-induced recurrent ulcerative colitis （UC） in rats and to probe into its underlying mechanism. METHODS： Recurrent colitis model was established in Wistar male rats by rectal ad- ministration of 80 mg/kg TNBS dissolved in 50% ethanol, and then the rats were second instilled with 30 mg/kg TNBS into the colon on the 16th day after the first induction UC. Rats were intragastrically administered different doses of GSPE （100, 200, and 400 mg/kg） per day for 7 days after twice-induction of colitis by TNBS. Sulfasalazine （SASP,500 mg/kg） was used as a positive control drug. Rats were killed after GSPE treatment 7 days. The colon sample was extracted, and the colon weight/length ratio （mg/cm） was assessed. The activities of, my- eloperoxidase （ MPO ）, superoxide dismutase （SOD）, glutathion peroxidase （GSH-Px）, inducible nitric oxide synthase （iNOS）, and the contents of malonyldialdehyde （MDA）and glutathione （GSH） and NO in serum were detected by biochemistry method. RESULTS： Compared with the model group, the rat body weight was decreased, and the colonic weight/length ratio was reduced in GSPE dosage group（P〈0.05 or P〈0.01）; the activities of MPO and iNOS and the contents of MDA,NO in serum were significantly decreased（P〈0.05 or P〈0.01） ; but the activities of MPO, iNOS and the contents of MDA, NO were decreased （P〈0.05 or P〈0.01） ; the activities of SOD, GSH-Px, the contents of GSH in serum were increased（P〈0.05 or P〈0.01）. CONCLUSION： GSPE can alleviate the inflammatory reactions in colitis through inhibiting oxygen free reaction, exerting antioxidation effects and decreasing the production of NO.

关 键 词：葡萄籽原花青素 三硝基苯磺酸 复发 大鼠结肠炎 抗炎 机制 

分 类 号：R965.2[医药卫生—药理学]