IL-6可增强猪卵巢颗粒细胞雄激素受体的表达  

IL-6 can upregulate porcine ovarian granulosa cells androgen receptor expression

作　　者：洪岭[1] 滕晓明[1] 李昆明[1] 黄文强[1] 张燕[2] 张博[2] 童晓文[2] 

机构地区：[1]同济大学附属第一妇婴保健院生殖医学中心,上海200040 [2]同济大学附属同济医院妇产科,上海200065

出　　处：《基础医学与临床》2010年第3期303-305,共3页Basic and Clinical Medicine

摘　　要：目的研究白细胞介素6(IL-6)对卵泡颗粒细胞雄激素受体(AR)表达的影响,探讨IL-6在多囊卵巢综合征(PCOS)发病机制中的作用。方法在体外培养的猪卵泡颗粒细胞培养液中分别加入10、100和1000ng/L的IL-6,采用实时荧光定量PCR和Western blot法观察IL-6对卵巢颗粒细胞ARmRNA和蛋白的表达。结果IL-6可上调卵巢颗粒细胞ARmRNA和蛋白的表达。结论IL-6增强雄激素的活性,可能参与了慢性亚临床炎症引发的PCOS。Objective To study the effect of interleukin 6 （IL-6） on the expression of androgen receptor （AR） in porcine ovarian granulosa cells（GC） and to explore its role of in the polycystic ovary syndrome（PCOS） pathogenesis.Methods Different concentrations of IL-6（10,100 and 1 000 ng/L） were treated with GC.Expression of androgen receptor （AR） mRNA and protein in GC were detected by real-time quantitative RT-PCR and Western blot.Results The mRNA and protein expressions of AR in GC were upregulated in groups with 100 and 1 000 ng/L IL-6 at 72 h.Conclusion IL-6 can improve androgen activity.This process may explain the function of chronic subclinical inflammation in PCOS pathogenesis.

关 键 词：IL-6 多囊卵巢综合征 卵泡颗粒细胞 雄激素受体 

分 类 号：R711.75[医药卫生—妇产科学]