肌肽对低氧所致大鼠血管内皮细胞损伤的保护作用  

Protective effect of carnosine on the injury of rat vascular endothelial cells induced by hypoxia

作　　者：白剑[1,2] 迟戈 张俊[4] 刘丽娜[1] 樊淼[1] 刘海东[1] 李伟伟[1] 杨菁[1] 

机构地区：[1]辽宁医学院生物化学与分子生物学教研室,锦州110021 [2]辽宁中医药大学,沈阳100032 [3]辽宁省食品药品监督管理局技术审评中心,沈阳110001 [4]大连医科大学解剖学教研室,辽宁大连116044

出　　处：《中国应用生理学杂志》2010年第1期30-32,共3页Chinese Journal of Applied Physiology

基　　金：辽宁省自然科学基金资助课题(20072202)

摘　　要：目的:研究肌肽对低氧所致大鼠血管内皮细胞损伤的影响。方法:建立低氧条件下大鼠血管内皮细胞损伤模型,用MTT法观察肌肽对低氧损伤的血管内皮细胞活性的影响,测定细胞培养基中LDH活力,并对细胞骨架进行考马斯亮蓝R-250染色观测其细胞结构。结果:浓度为10mmol/L～20mmol/L肌肽孵育血管内皮细胞6h后,可以抑制缺氧12h和24h引起的血管内皮细胞活性下降,同时减少LDH的释放,保持细胞骨架完整。结论:肌肽对低氧所致的血管内皮细胞损伤具有保护作用。Objective：To investigate the effect of camosine on the injury of rat vascular endothelial cells（VECs） induced by hypoxia.Methods：The model of the injury of rat VECs induced by hypoxia was established.The effect of camosine on injury of VECs activity induced by hypoxia was determined by MTT assay.The levels of lactate dehydrogenase （LDH） activity in cell medium were measured with correspon-ding kit.The cell structure was observed under microscope after Coomassie brilliant blue R-250 staining.Results：After culturing VECs with camosine （10 to 20 mmol/L ） for 6 hours,the decrease in VECs activity induced by 12 and 24 hour hypoxia was inhibited.The release of LDH was also inhibited,and the integrity of cell structure reamained.Conclusion：Camosine has the protective effect on hypoxia injured VECs.

关 键 词：肌肽 细胞骨架 血管内皮细胞损伤 缺氧 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]