蓝莓提取物对H_2O_2诱导的体外培养的大鼠海马神经元氧化损伤的保护作用  

Cytoprotective effect of blueberry extracts against oxidative damage of rat hippocampalneurons induced by H_2O_2

作　　者：庞伟[1] 蒋与刚[1] 杨红澎[1] 程道梅[2] 卢豪[2] 卢士军[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050 [2]四川大学华西公共卫生学院,成都610041

出　　处：《中国应用生理学杂志》2010年第1期51-54,共4页Chinese Journal of Applied Physiology

基　　金：天津市应用基础及前沿技术研究计划资助项目(09JCYBJC12900);中国营养学会营养科研基金项目(2004-09)

摘　　要：目的:探讨蓝莓提取物对过氧化氢致大鼠海马神经元氧化应激损伤的减缓作用。方法:将培养7d的海马神经细胞分为8组:①过氧化氢组(H2O2):培养液中加入50μmol/L的H2O2,作用24h。②不同剂量蓝莓提取物预处理组(BE+H2O2):在加入H2O2前24h,分别加入0.01、0.1、1.0、10.0、20.0和40.0μg/mlBE。③空白对照组(Control):处理步骤同上组,但每次处理物质均为等量的培养液。通过测定细胞存活率和上清液中乳酸脱氢酶(LDH)的活性确定减轻海马神经元损伤的蓝莓提取物的适宜浓度。并检测细胞内丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性以及细胞凋亡率的变化。结果:①蓝莓提取物组(0.1,1.0和10.0μg/mlBE)LDH活性显著低于H2O2组,细胞存活率由H2O2组的57.44%分别上升至78.42%、87.71%、72.40%;1μg/ml蓝莓提取物组对H2O2诱导的海马神经细胞氧化应激损伤的保护作用最好。②1μg/ml蓝莓提取物组海马神经细胞培养上清液中MDA含量及细胞凋亡率显著低于H2O2组,SOD活性显著高于H2O2组。结论:适宜剂量的蓝莓提取物对氧化应激损伤的海马神经元有一定的保护作用,其机制可能与抑制海马神经元凋亡、增强神经细胞的抗氧化功能有关。Objective：To investigate the protective effect of blueberry extract （BE,25% anthocyanins） against oxidative damage in primary cultures of rat hippocampal neurons induced by H2O2.Methods：Rat hippocampal neurons were randomly assigned to control group,H2O2 group and BE pretreatment groups,BE at six different doses（0.01、0.1、1.0、10.0、20.0 and 40 μg/ml） and then exposed to 50 μmol/L H2O2 for twenty-four hours.To selecte the most fittest concentration of BE by testing viability of neurons and activity of LDH.Then MDA concentration,SOD activity and neuronal apoptosis were（checked） measured.Results：①Compared with H2O2 group,the hippocampal cell viabilities in the 0.1,1.0 and 10 μg/ml BE groups were significantly increased from 57.44% to 78.42%,87.71% and 72.40% separately.The activity of LDH in BE groups at varied concentrations（0.1、1.0 and 10 μg/ml） was significiantly lower than that in H2O2 group.It was found that 1 μg/ml BE had the furthest protective effect against oxidative damage in primary cultures of rat hippocampal neurons induced by H2O2.②The concentration of MDA and the rate of neuronal apoptosis of BE group（1 μg/ml） were much lower than H2O2 group,while SOD activity was much higher.Conclusion：Proper dose of BE has remarkable protective effect against oxidative stress in primary cultures of rat hippocampal neurons induced by H2O2,the mechanism may be related to decreasing the neuronal apoptosis and enhancing the antioxidation of hippocampal neurons.

关 键 词：海马神经元 氧化应激 蓝莓提取物 

分 类 号：R965.1[医药卫生—药理学]