溶血卵磷脂对人内皮细胞CD73的调节与PKC的关系  

Role of PKC in regulation of CD73 by lysophosphatidylcholine in human endothelial cells

作　　者：张群英[1] 张华[1] 谭劼[1] 韩君勇[1] 

机构地区：[1]中山大学附属第五医院,广东珠海519000

出　　处：《中国应用生理学杂志》2010年第1期102-104,共3页Chinese Journal of Applied Physiology

基　　金：广东省医学科研基金(A2008231);珠海市科技项目(20081084)

摘　　要：目的:探讨溶血卵磷脂(LPC)对人脐静脉内皮细胞(HUVEC)胞外5’-核苷酸酶(CD73)的调节作用及其与蛋白激酶C(PKC)的关系。方法:将含已融合HUVEC的细胞培养皿分为4组(n=15):①LPC组:培养皿中加入LPC10μmol/L;②CHE(白屈菜赤碱,PKC抑制剂)组:加入CHE100μmol/L及LPC10μmol/L;③AOPCP(α,β-甲基腺苷-5′-二磷酸,CD73抑制剂)组:加入AOPCP50μmol/L及LPC10μmol/L;④对照组:无干预。每组均于实验开始时加入乙烯-单磷酸腺苷(eAMP,5μmol/L)。在实验第15、30、45min测定各培养皿中乙烯-腺苷(eAD)含量。结果:在上述三个时间点LPC组HUVEC的eAD生成均较对照组显著增高(P<0.05),加入CHE使LPC的这种增加作用消失,其eAD生成与对照组无差别(P>0.05),而AOPCP组eAD生成较其它三组均显著减少(P<0.01)。结论:LPC可兴奋内皮细胞胞外CD73,此作用可被PKC抑制剂CHE抑制,LPC上调CD73活性作用可能与PKC有关。Objective：To discuss the effect of protein kinase C （PKC） on regulation of ecto-5＇-nucleotidase activity by lysophosphatidylcholine（LPC） in human umbilical endothelial cells （HUVEC）.Methods：Experiments were conducted in HUVEC grown on dishes which were divided into 4 groups （n=15）：①Control group in which only eAMP（5 μmol/L） was added;②LPC group in which HUVEC were incubated with LPC（10μmol/L） before eAMP was added;③Chelerythrine group in which cells were pre-incubated with the PKC inhibitor chelerythrine（100μmol/L） before LPC and eAMP were added;④α,β-Methyladenosine-5＇-Diphosphate（AOPCP） group in which cells were incubated with AOPCP （10μmol/L） before eAMP was added.Etheno-adenosine production was detected at 15th,30th,45th min with high performance liquid chromatography（HPLC） respectively.Results：Comparing to the control group LPC significantly increased etheno-adenosine production at three time points respectively（P〈0.05）.Furthermore,PKC inhibitor chelerythrine abolished this effect of LPC and the etheno-adenosine production at three time points were at the same level of control group （P〉0.05）.CD73 inhibitor AOPCP significantly decreased the etheno-adenosine production compared to the other three groups（P〈0.01）.Conclusion：Ecto-5＇-nucleotidase can be modulated within minutes following exposure of HUVEC to LPC and this response may be mediated by PKC in HUVEC.

关 键 词：腺苷 内皮细胞 胞外5′-核苷酸酶 

分 类 号：R333.6[医药卫生—人体生理学] R321.2[医药卫生—基础医学]