深黄被孢霉脂质提取物改善大鼠脂质肾损害  

Mortierella isabellina lipid-soluble extracts improves lipid-induced renal injury in rats

作　　者：张玲[1] 曹丹[1] 李敏[2] 

机构地区：[1]重庆医科大学附属第二医院肾内科,重庆400010 [2]福建师范大学生命科学学院,福州350007

出　　处：《中国应用生理学杂志》2010年第1期121-124,I0003,共5页Chinese Journal of Applied Physiology

基　　金：重庆市教委科研基金项目(KJ050312)

摘　　要：目的:探讨深黄被孢霉脂质提取物能否改善脂质肾损害模型大鼠血脂异常及肾脏损伤。方法:雄性Wis-tar大鼠随机分为4组(n=8):①对照(N)组;②高脂模型(M)组;③深黄被孢霉脂提物干预(T)组;④辛伐他汀治疗(S)组。N组饲以普通饲料,M组给予高脂饲料喂养,两组均每日灌服生理盐水;T组和S组在高脂饲料喂养同时每日分别灌服深黄被孢霉脂提物和辛伐他汀。于实验前、第4、8、12周检测24h尿蛋白含量,12周时检测血清白蛋白、总蛋白、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、尿素氮(BUN)、肌酐(Scr)水平;检测肾组织TG、TC、LDL含量;观察肾组织病理及超微结构改变、脂质沉积。结果:实验第12周,T组和S组24h尿蛋白定量、血清及肾组织TC、LDL明显低于模型组,血清白蛋白、总蛋白显著高于模型组,T和S组之间差异无显著性。病理改变显示:M组肾小管间质大量炎症细胞浸润,管腔内有蛋白管型,肾小球系膜细胞轻度增生、系膜基质增宽,T组与S组炎症细胞浸润明显减少,未见蛋白管型及系膜区病理改变;M组肾小球及肾小管内大量脂肪沉积,足细胞足突融合消失;T组与S组未见脂肪沉积;足细胞仅部分足突融合,小管上皮细胞内仅少量脂滴。结论:深黄被孢霉脂质提取物能改善高脂模型大鼠的高胆固醇血症,减少肾组织中胆固醇的沉积、炎症细胞的浸润及足细胞的损伤,减少尿蛋白漏出,对脂质肾损害大鼠肾脏具有保护作用。Objective：To investigate the effect of the Mortierella isabellina lipid-soluble extracts on improving hyperlipidemia and renal damage in lipid-induced renal injury rats.Methods：Thirty two male Wistar rats were divided into four groups：normal group（N）,model group（M）,the group treated with Mortierella isabellina lipid-soluble extracts（T） and Simvastatin（S）.N group was fed a standard diet and the other groups were fed a high-fat diet.T and S groups were orally administered by Mortierella isabellina lipid-soluble extracts and Simvastatin respectively for 12 weeks.N and M groups were orally administered by the normal saline.Twenty four-hour urinary protein excretion was detected.The levels of serum ALB,TP,BUN,Scr,TG,TC,LDL and TG,TC,LDL in the kidney were measured at week 12.The histological change,ultrastructural change and lipid deposits in kidney were evaluated.Results：At the 12th week,compared with M group,twenty four-hour urinary protein excretion and the levels of TC,LDL in serum and renal tissue in T and S group decreased （P〈0.01 or P〈0.05）,but the levels of ALB and TP in T and S group increased markedly（P〈0.01）.There was no significant difference of the levels of BUN,Scr,and TG among the four groups.The protein casts in renal tubules,inflammatory cell infiltration in tubulointerstitium as well as mild mesangial cell proliferation and expanded mesangial matrix were obseverd in M group.Electron micrograph demonstrated massive fusion of foot process of podocytes accompanied with rich lipid droplets in proximal tubular epithelial cells in M group.Conclusion：The lipid-soluble extracts of Mortierella isabellina has beneficial effect on improving hypercholesterolemia and kidney damage in lipid-induced renal injury rat.

关 键 词：深黄被孢霉脂质提取物 高脂血症 肾损害 大鼠 

分 类 号：R322.6[医药卫生—人体解剖和组织胚胎学]