广东地区副猪嗜血杆菌KRG血清分型及基因分型多态性分析  

KRG Serotyping and genotying of Haemophilus parasuis isolates from Guangdong Province

作　　者：贾爱卿[1] 李春玲[1] 王贵平[1] 杨冬霞[1] 何丽丽[1] 

机构地区：[1]广东省农业科学院兽医研究所,广东省兽医公共卫生公共实验室,广州510640

出　　处：《农业生物技术学报》2010年第1期103-107,共5页Journal of Agricultural Biotechnology

基　　金：广东省农业攻关项目(No.2007A020300005-11)资助

摘　　要：本研究对保存的2007～2008年广东地区发病猪群中分离的48株副猪嗜血杆菌(Haemophilus parasuis)进行了分析。用琼脂扩散进行血清分型,结果表明48株临床分离菌株鉴定出7个血清型,其中以5型为主,占总菌株的23.68%。采用肠杆菌基因间重复序列-PCR(Enterobacterial repetitive intergenic consensus-PCR)法进行基因分型分析,结果显示,48株不同来源副猪嗜血杆菌菌株基因型呈现多态性分布;分析得到31个基因型PHYLIP-3.68软件对31个基因型的电泳图谱进行基因分型及遗传关系的分析,结果得到8个群,其中B为优势群,占总菌株数的66.67%。表明广东地区的副猪嗜血杆菌的基因型和血清型十分丰富,ERIC-PCR技术可成为其分子流行病学研究的有效手段。The total 48 Haemophilus parasuis strains isolated from Guangdong province in 2007～2008 were serotyped by Kieletein-Rapp-Gabried-son,and respectively belonged to 7 serotypes,and the main serotype is 5 with 23.68%.Furthermore,the genotypes and phylogenetic relationship of all isolates were analysed by PHYLIP-3.68 after Enterobacterial Repetitive Intergenic Consensus-PCR（ERIC-PCR）,the results indicated that the 48 isolates distributed to 31 genotypes among 8 clusters,especially B cluster with 66.67%.The findings suggested that the genetic polymorphism and serotypes of H.parasuis parasuis isolates are diverse in Guangdong province,and ERIC-PCR is an effective method of researching molecular epidemiology of animal infective diseases.

关 键 词：副猪嗜血杆菌 血清分型 ERIC-PCR 基因分型 

分 类 号：S858.28[农业科学—临床兽医学]