烟碱微透析回收率的体外测定方法及影响因素研究  

Study on the In-vitro Microdialysis Recovery of Nicotine and Its Influence Factors

作　　者：陈翔[1] 凌家俊[1] 陈亮[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《广州中医药大学学报》2010年第2期159-163,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:30772791)

摘　　要：【目的】研究利用浓差法中的减量法(即体内研究中的反向透析法)测定烟碱微透析回收率的可行性,并考察回收率测定中的相关影响因素,为烟碱的体内微透析研究提供实验依据。【方法】分别采用浓差法(增量法、减量法)测定烟碱的体外回收率,考察灌流液预处理及流速、浓度对其影响,并考察回收率的稳定性。【结果】滤过、搅拌能有效提高体外回收率,超声时间对回收率影响不大;增量法及减量法测定的回收率一致;回收率与媒介中烟碱的浓度无关,稳定性良好。【结论】实验时有必要滤过灌流液,对灌流液进行超声或以其他方式脱气。反向透析法可作为体内微透析研究中烟碱回收率的测定方法。Objective To detect the in-vitro microdialysis recovery of nicotine by decrement method （i. e. retrodialysis） and to investigate its influence factors, thus to supply experimental evidence for the in-vivo mierodialysis of nicotine. Methods The in-vitro recovery of nicotine was detected by concentration difference method （ increment method and decrement method）. The stability of recovery was observed. The effects of the pretreatment with perfusate, and the flow rates and concentrations of the perfusate on nicotine recovery were studied. Results Filtering and stirring treatment increased the recovery, but the time of ultrasound degassing showed no effect on the recovery. The recovery detected by increment method was as the same as that detected by decrement method. Nicotine recovery was independent of nicotine concentration in the external medium, showing good stability. Conclusion It is necessary to filter and degas the perfusate before microdialysis. Retrodialysis can be used for the determination of in-vivo nicotine recovery.

关 键 词：烟碱/分析 微透析 色谱法 高压液相 

分 类 号：R284.1[医药卫生—中药学]