HPLC-ELSD测定不同产地路路通药材中路路通酸的含量  

HPLC-ELSD determination of acid in fiquidambris from various habitats

作　　者：刘菁[1] 李志浩[1] 郑芳[1] 朱雪松[1] 孙新建[1] 何婧[1] 陈银华[2] 

机构地区：[1]郧阳医学院附属东风医院药剂部 [2]湖北十堰市药品检验所,湖北十堰442000

出　　处：《安徽医药》2010年第3期286-287,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的考察不同产地路路通中路路通酸的含量。方法路路通样品经过无水乙醇超声处理后，采用HPLC—ELSD测定不同产地路路通中路路通酸的含量，Diamonsil C18色谱柱，以甲醇-0．7％醋酸溶液（85：15）为流动相，流速1．0min^-1，柱温30℃，漂移管温度45℃，载气为N2，流速3．5min^-1采用外标两点法计算含量。结果路路通酸在0．058—0．580g·L^-1浓度范围内有良好线性关系（r=0．9997），平均加样回收率为98．9％，RSD为1．25％。结论各产地路路通中路路通酸含量有明显的差异，湖北各地路路通有较高的路路通酸，有开发利用的价值。Aim To determine the liquidambaric acid in Fructus Liquidambris. Method Fructus Liquidambris was extracted in EtOH by microwave. The liquidambaric acid was determined by HPLC -ELSD. Diamonsil C18 column was used, the mobile phase consisting of methanol - 0.7% acetic acid （ 85 ： 15 ）. The flow rate was 1.0 min^-1. The column temperature was 30℃ , and the temperature of drift tube was 45℃. Nitrogen gas as carrier gas, the flow rate was 3.5 ml·min^-1 ,with a universal detector evaporation light scattering detector （ELSD）. Contents were calculated with external standard two-point method. Results The linear response range of liquidambaric acid was from 0. 058 to 0. 580 g·L^- 1 （ r = 0. 9997 ）. The average recovery was 98.9% , and RSD was 1.25%. Conclusion There are great differences among different originations. The content of liquidambaric acid in Hubei fructus liquidambris is the most,which was valuable for further exploitation and application.

关 键 词：路路通 路路通酸 高效液相色谱-蒸发光散射法 含量测定 

分 类 号：R284.1[医药卫生—中药学]