高效液相色谱法测定酚酞栓的含量  

Determination of phenolphthaleinum in phenolphthalein suppository by HPLC

作　　者：董艳[1] 

机构地区：[1]安徽省淮北矿工总医院,安徽淮北235000

出　　处：《安徽医药》2010年第3期299-300,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立高效液相色谱测定酚酞栓中酚酞含量的方法。方法色谱柱以十八烷基硅烷键合硅胶为填充剂；甲醇-水（60：40）为流动相；检测波长为275nm。结果线性关系为Y=15．8649X＋3．8656，r=0．9999，线性范围在10．035～401．400μg，平均回收率104．99％（n=9），RSD=1．09％。结论本法操作简便准确，可作为酚酞栓中酚酞含量的质量控制方法。Aim To establish a method of determination of phenolphthaleinum in phenolphthalein suppository by HPLC. Methods For chromatographic column ODS-C18 was used as the filling agent. The flowing phase was methyl alcohol-water（ 60： 40） , and the examination wave length was 275 nm. Results The linear relationship was Y = 15. 8649X ＋ 3. 8656, r = 0. 9999. The linear range was from 10. 035 to 401. 400 μg. The average recovery was 104.99% （n = 9） ,with an RSD of 1.09%. Conclusion The method was simple, accurate, and could be used for the determination of Phenolphthaleinum in Phenolphthalein Suppository.

关 键 词：高效液相 酚酞栓 含量 

分 类 号：R927[医药卫生—药学]