Toll样受体4在感染性早产患者外周血单个核细胞的表达及意义  

Expression and significance of Toll-like receptor 4 in mononuclear cells of peripheral blood in infected patients of premature labor

作　　者：董生凤[1] 罗艳[2] 王豫黔[2] 

机构地区：[1]遵义医学院附属医院妇产科,贵州遵义563003 [2]遵义医学院第五附属(珠海)医院妇产科

出　　处：《中国妇幼保健》2010年第8期1088-1091,共4页Maternal and Child Health Care of China

摘　　要：目的:观察感染性早产患者外周血单个核细胞Toll样受体4(TLR4)的表达变化,探讨其与感染性早产发生的关系以及在早期预测中的价值。方法:收集31例早产(28～36+6周)患者部分胎盘胎膜组织行病理检查,根据病检有无绒毛膜羊膜炎分为早产感染组(17例)和早产非感染组(14例),门诊进行孕检的同孕周正常孕妇作为正常对照组(10例)。①采用酶联免疫吸附法测定血清中的IL-6水平;②应用流式细胞术观察早产患者和正常对照组孕妇外周血CD14+单个核细胞上TLR4分子的表达率和平均荧光强度(MFI);③检测血常规。结果:①TLR4在早产感染组患者外周血CD14+单个核细胞的阳性率为(39.22±13.17)%,平均荧光强度为(48.45±11.66),显著高于早产非感染组和正常对照组(P<0.05);而TLR4分子在早产非感染组和正常对照组外周血CD14+单个核细胞的阳性率和MFI相比,差异无统计学意义(P>0.05);②早产感染组孕妇血清中IL-6水平为(2.56±0.36)pg/ml,明显高于早产非感染组孕妇和正常对照组孕妇,差异有统计学意义(P<0.05),但早产非感染组和正常对照组血清中IL-6水平比较,差异无统计学意义(P>0.05);③感染性早产患者外周血TLR4的MFI与血清中IL-6水平成正相关(r=0.727,P<0.05),与血常规中的白细胞总数和中性粒细胞百分比无明显相关性。结论:①感染性早产患者外周血单个核细胞表面TLR4及血清IL-6表达明显增强,提示TLR4的信号通路可能与感染性早产的发生发展密切相关;②TLR4平均荧光强度与血清中IL-6水平成正相关,可作为早期预测早产宫内感染的指标。Objective： To observe the changes of Toll - like receptor 4 （TLR4） expression in mononuclear cells of peripheral blood, explore the relationship between TLR4 and infectious premature labor and its significance in predicting premature labor. Methods： Placental tissues and fetal membranes from 31 patients with premature labor （gestational weeks： 28 - 36^ ＋6 weeks） were collected for pathological examination, then the patients were divided into infected group （ 17 cases） and non - infected group （ 14 cases） according to chorioamnionitis or not, and 10 normal pregnant women in the same gestational weeks were selected as control group. The serum level of IL -6 was detected by ELISA; the expression rate and mean fluorescence intensity （MFI） of TLR4 in mononuclear cells of peripheral blood in patients with premature labor and control group were detected by flow eytometry; blood routine examination was carried out. Results： The positive rate and M FI of TLR4 in mononuclear ceils of peripheral blood in infected group were （39. 22 ± 13.17 ） % and （48.45 ± 11.66）, respectively, which were significantly higher than those in non - infected group and control group （P 〈 0. 05 ） ; there was no significant difference in the positive rate and MFI of TLR4 in mononuclear cells of peripheral blood between noninfected group and control group （P 〉 0.05 ） . The serum level of IL - 6 in infected group was （ 2. 56 ± 0. 36 ） pg/ml, which was significantly higher than those in non - infected group and control group （P 〈 0. 05）, but there was no significant difference in serum level of IL - 6 between non - infected group and control group （P 〉0. 05） . There was a positive correlation between MFI of TLR4 in mononuclear ceils of peripheral blood and serum level of IL - 6 in infected group （ Y = 0. 727, P 〈 0. 05 ）, but there was no correlation between MFI of TLR4 in mononuclear cells of peripheral blood and total number of leucocytes, percentage of neutrophile granulocy

关 键 词：TOLL样受体4 早产 宫内感染 流式细胞术 

分 类 号：R714.21[医药卫生—妇产科学]