中药复方制剂对大肠埃希菌多重耐药基因AcrA-mRNA表达水平的影响  

Influence of Chinese Traditional Compound Medicine on Escherichia coli Gene AcrA-mRNA by Real-time PCR

作　　者：任玲玲[1] 鞠玉琳[1] 平家奇[1] 张宇[1] 

机构地区：[1]延边大学农学院,吉林龙井133400

出　　处：《湖北农业科学》2010年第2期257-259,共3页Hubei Agricultural Sciences

基　　金：吉林省教育厅"十一五"科学技术研究项目(吉教合字[2006]第4号)

摘　　要：研究中药复方制剂连黄(精提、粗提)对大肠埃希菌多重耐药基因AcrA的mRNA表达水平的影响,从而深入探讨中药复方制剂连黄(精提、粗提)抑制大肠埃希菌多重耐药性的作用机理。采用实时荧光定量PCR法,定量分析了中药复方制剂连黄(精提、粗提)作用前后的大肠埃希菌耐药基因AcrA的mRNA表达水平。结果表明,精提和粗提的中药复方制剂均能降低AcrA基因的mRNA表达水平,且精提制剂较粗提制剂对AcrA基因的mRNA表达水平影响更大。The influence of Chinese traditional compound medicine on mRNA of Escherichia coli gene AcrA was studied to probe the mechanism of action. The expression of E. coli gene AcrA-mRNA was determined by real-time PCR before and after the using of Chinese traditional compound medicine. The results showed that crude and refined Chinese traditional compound medicine could reduce the expression of AcrA-mRNA, and the refined Chinese traditional compound medicine drug-resistance gene decreased after by dealing with the crude and refined Chinese traditional compound medicine had greater impact on AcrA-mRNA.

关 键 词：实时荧光定量PCR 中药复方制剂连黄 大肠埃希菌 AcrA—mRNA 

分 类 号：Q503[生物学—生物化学] S853.7[农业科学—临床兽医学]