毒芹诱导分化培养基筛选  

Selection of Differential Medium for Cicuta virosa

作　　者：王艳玲[1] 李云霄[1] 

机构地区：[1]吉林农业科技学院,吉林吉林132100

出　　处：《湖北农业科学》2010年第2期283-284,共2页Hubei Agricultural Sciences

基　　金：吉林省教育厅科研项目(吉教科合字[2006]167号)

摘　　要：通过8种添加不同种类和不同浓度植物激素的培养基,对毒芹进行组织培养试验,研究了不同种类和不同浓度植物激素对毒芹诱导成活数的影响,筛选出较适合诱导毒芹分化的培养基。结果表明,MS+6-BA 2mg/L+NAA 0.3mg/L+2,4-D 0.5mg/L+GA3 0.7mg/L、MS+6-BA 2mg/L+NAA 0.3mg/L+2,4-D 0.7mg/L和MS+6-BA 2mg/L+NAA 0.5mg/L+2,4-D 0.5mg/L培养基对毒芹的诱导成活率较高。是适合毒芹诱导分化的培养基。The survival techniques of induction of Cicttta virosa was studied by using tissue culture methods and adopting MS basic medium,adding the different types and different concentrations of plant hormones, choosing non-polluting dedicate stem Cicuta virosa, then carrying out random trial of tissue culture. According to the trial results, E （MS＋NAA0.3＋2,4- DO.5mg/L＋GA3 0.Tmg/L）,F（MS＋NAA0.3 mg/L＋2,4-D0.Tmg/L）,G（MS＋NAAO.5mg/L＋2,4-DO.5mg/L）had higher survival rate of induction.

关 键 词：毒芹 组织培养 NAA 2 4-D GA3 

分 类 号：Q949.763.3[生物学—植物学] Q813.12