肉桂酸和不同光质对葡萄愈伤组织增殖及白藜芦醇累积的效应  

Effects of cinnamic acid and different light quality on callus multiplication and resveratrol accumulation in callus from Vitis vinifera

作　　者：刘媛[1] 李胜[1,2] 马绍英[1,2] 张真[1] 薛冲[1] 罗丽媛[1] 张青松[1] 

机构地区：[1]甘肃农业大学生命科技学院细胞工程实验室,甘肃兰州730070 [2]兰州汇通生物科技有限公司,甘肃兰州730000

出　　处：《甘肃农业大学学报》2010年第1期41-46,共6页Journal of Gansu Agricultural University

基　　金：国家星火项目(2008GA860007);甘肃省科技支撑计划(0804NKCA081);甘肃省科技攻关项目(2GS064-A41-002-09);科技部中小企业创业基金(06C26226201858);甘肃省高校研究生导师项目(0602-01)

摘　　要：以葡萄品种"黑比诺"叶片诱导出的愈伤组织为材料,研究了不同浓度的肉桂酸和不同光质对葡萄愈伤组织增殖及对其中白藜芦醇合成的影响.结果表明:肉桂酸浓度为21mg·mL-1时,白光有最利于葡萄愈伤组织的增殖;在肉桂酸浓度为27mg·mL-1时,绿光最有利于葡萄愈伤组织的增殖;白藜芦醇含量在肉桂酸浓度为27mg·mL-1的白光下最高,但产量却在蓝光下最高;葡萄愈伤组织采收后经紫外强度8μmol.m-2.s-1处理10min可显著提高其中的白藜芦醇含量;而当肉桂酸与紫外两个因素共同作用时对白藜芦醇合成的促进作用不如两因素单独作用时的强.The callus cultured from the grape leaves（cv.Pinot Noir）were used in the experiment to study the effects of cinnamic acid（CA）at different concentrations and different light quality on callus multiplication and resveratrol（Res）production.The results showed that CA at 21 mg·L-1 under white light and 27 mg·L-1 under green light were beneficial to callus multiplication.The treatment of CA at 27 mg·L-1 under white light benefited to Res accumulation;however,blue light was optimal to Res production.The treatment at 8 μmol·m-2·s-1 of UV-C for 10 min promoted Res content of callus significantly.The combined action of UV-C and CA was inferior to its single.

关 键 词：葡萄 白藜芦醇 愈伤组织 肉桂酸 光质 增殖倍数 

分 类 号：S663.1[农业科学—果树学]