藏红花内生真菌的分离和代谢产物的初步研究  

The initial research on osolation and metabolism of endophytic fungus from Crocus sativus L.

作　　者：焉兆萍[1] 李永乐[1] 赵军[1] 徐德强[2] 肖义平[2] 裴卫忠 

机构地区：[1]上海师范大学生命与环境科学学院 [2]复旦大学生命科学院微生物和微生物工程系 [3]上海华宇药业有限公司

出　　处：《上海师范大学学报（自然科学版）》2010年第1期71-77,共7页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海师范大学校基金(SK200712);上海市教委项目(09YZ155)

摘　　要：通过不同灭菌处理方式和4种不同培养基培养,从健康藏红花植株体内分离得到63株内生真菌.其中,球茎中分离到60株内生真菌,花药中分离到2株,柱头中分离到1株,花被、嫩叶、芽鞘、花柱中均未分离到内生真菌.4种培养基的筛选结果表明:高盐察氏培养基相对适合于藏红花内生真菌的分离.通过对4种病源指示菌的生长抑制试验,筛选出12株内生真菌对病原菌有一定程度的抑制作用.通过对分泌物产生菌的代谢产物分析研究,发现4株特异性代谢黄酮类或皂甙类物质的生产菌.After sterilized with different methods followed by culture on four kinds of media, sixty - three endophytic fungal strains were isolated from healthy Crocus sativus L. , among which sixty from bulbs, two from anthers, one from stigmas. However no fungal strains were isolated from perianth, leaves, coleoptiles and styles. The screening results for culture mediums show that the high -salt czapeks^edium is suitable for culture of endophytic fungus. The antifungal activity screening with four microbe show that twelve endophytic fungal strains have the capacity to inhibit at least one plant pathogen to different extent. The metabolite analysis of secrete and strain lysis show that four strains had preponderant metabolites related flavonoids or saponins.

关 键 词：藏红花 内生真菌 分离 代谢产物 

分 类 号：G94-334[文化科学]