黄瓜抗枯萎病相关基因cDNA的分离及鉴定  

Isolation and Identification of Resistance-related Genes for Fusarium Wilt in Cucumber

作　　者：沈凤瑞[1,2] 吴萍[1] 毛爱军[1] 周红梅[1] 王永健[1] 于拴仓[1] 张丽蓉[1] 杨艳丽[2] 郑晓鹰[1] 

机构地区：[1]北京市农林科学院蔬菜研究中心,北京100097 [2]云南农业大学,云南昆明650201

出　　处：《华北农学报》2010年第1期40-43,共4页Acta Agriculturae Boreali-Sinica

基　　金：北京市自然科学基金(5062009);国家"863"计划(2006AA100108);北京市外包项目(Z07080501570702);北京市优秀人才(20081D0200500054);北京市重点课题(D08070500690803);国家科技支撑计划(2008BADA1B05)

摘　　要：黄瓜枯萎病是造成黄瓜减产的主要病害之一,研究黄瓜枯萎病抗性分子机理对黄瓜抗病育种有重要意义。以黄瓜抗枯萎病材料Cu14为试材,采用基因差异显示技术(DDRT-PCR)研究黄瓜接种枯萎病生理小种4后基因的表达情况,分离出1个接种后在根茎部特异表达的差异cDNA片断,命名为A178-2。A178-2基因片段的328个核苷酸中有145个核苷酸与拟南芥泛素蛋白同源,氨基酸序列同源性达到85%。Fusarium wilt（Fusarium oxysporum f.sp.cucumerinum Owen. is one of major diseases that cause significant disease losses in cucumber.It is significant to explore the disease resistance molecular mechanism against Fusarium wilt race 4 in cucumber breeding.The cucumber resistant line Cu14 were employed for studying on resistant genes expressing against Fusarium wilt by differential display reverse transcriptase PCR（DDRT-PCR） method.One fragment of cDNA expressed specially was got from the root-stem inoculated in cucumber,and was named as A178-2 with the size of 328 bp.Blast analysis showed that 145 bp nucleotide sequences of A178-2 was homologous with the generic grain protein in Arabidopsis and reached 85%.Further studying on expression of the gene will be done.

关 键 词：黄瓜 枯萎病生理小种4 抗病相关基因cDNA 分离 鉴定 

分 类 号：Q74[生物学—分子生物学] S642.2[农业科学—蔬菜学]