RAPD多态位点数变化对小豆遗传变异信息估计的影响  

The Influence of Vary Number of RAPD Polymorphic Loci to Information Evaluation of Genetic Variation in Adzuki Bean

作　　者：文自翔[1,2] 金文林[1] 濮绍京[1] 赵波[1] 卢为国[2] 李海朝[2] 李金英[2] 

机构地区：[1]北京农学院作物遗传育种研究所,北京市农业应用新技术重点实验室,北京102206 [2]河南省农业科学院经济作物研究所,河南郑州450002

出　　处：《华北农学报》2010年第1期75-79,共5页Acta Agriculturae Boreali-Sinica

基　　金：北京市属市管高等学校人才强教计划项目((PXM2006-014207-021717);现代农业产业技术体系建设专项资金资助

摘　　要：以163份来自于中国、日本、韩国、不丹、尼泊尔和越南6国的各种类型小豆种质资源的大样本群体为材料,利用27条RAPD引物检测该群体得到的261个等位基因数据构建成＂总群体＂,采用完全随机抽样方法,研究不同样本容量（样本位点数）与系统遗传相似系数（Jaccard系数）变化的关系,并进一步配置出不同引物数量、多态位点与Jaccard系数的变异系数曲线方程,通过分析回归曲线的相应切点,确定在一定精度下适合的引物数量及多态位点数。研究结果表明：不同引物数量之间的Jaccard系数的标准差、平均数、变异系数差异极显著。不同引物数量、不同多态位点与Jaccard系数的变异系数、标准差回归方程为Y=a＋b/X形式,决定系数均在0.95以上。RAPD遗传信息分析精度控制在5%-10%时的最适样本容量为15-32条随机引物或120-230个多态性位点。RAPD（Random amplified polymorphic DNA） variation was assessed in 163 accessions of adzuki bean from China,Japan,Korea,Bhutan,Nepal and Vietnam.A ＂population＂ was constructed by 261 polymorphic loci detected by 27 RAPD primers.The method of entirely random sampling was adopted to analyze the regression relationship between the sizes of sample（allelic number） and genetic similarity index（Jaccard index）.Regression equation for different number of primers,loci and Jaccard index was calculated out,and then the reasonable number of primers and loci under given precision can be decided by analyzing the tangent of the curve equation.The results were as follows：There exists significant difference among the S,CV and average of Jaccard index calculated from different number of primers.Regression equation for different number of primers,loci and the CV of Jaccard index was Y=a＋b/X,and the determine index was above 0.95.Under the precision of 5%—10%,the reasonable sample should be 15—32 RAPD primers or 120—230 polymorphic loci.

关 键 词：小豆 遗传相似系数 RAPD 多态位点数 

分 类 号：S521[农业科学—作物学]