提莫菲维小麦与葡萄牙野燕麦远缘杂交后代的SRAP分析  

Analysis of the Progeny from Wide Cross T.timopheevi and Avena fatua L. var.Portugal Using SRAP Technique

作　　者：耿广东[1] 张素勤[1] 贾开家[1] 徐如宏[1] 任明见[1] 张庆勤[1] 

机构地区：[1]贵州大学农学院,贵州贵阳550025

出　　处：《华北农学报》2010年第1期110-112,共3页Acta Agriculturae Boreali-Sinica

基　　金：教育部春晖计划(15001D-3);贵州省优秀科技教育人才省长基金(黔省专合字(2007)19);贵州省农业攻关项目(黔科合NY字(2006)3002B;黔科合NY字[2008]3017号;黔科合带帽字([2009]5002)

摘　　要：采用SRAP技术分析提莫菲维小麦与葡萄牙野燕麦远缘杂交后代的真实性及其特点。结果表明,22对SRAP引物中,20对引物在双亲间扩增出多态性条带,其多态性比率为73.81%。me4-em1、me3-em5、me4-em3和me3-em3四对引物在提莫菲维小麦与葡萄牙野燕麦杂交F3株系中扩增出双亲特异带,表明该F3株系具有双亲的遗传物质,该F3株系是提莫菲维小麦和葡萄牙野燕麦成功属间杂交的真实杂种后代。在F3株系的扩增结果中部分双亲带型消失,并且提莫菲维小麦消失的带数远少于野燕麦的;同时有非父母标记新带型出现。杂种后代DNA序列的这种变化可能有利于新形成异源多倍体小麦的快速进化、遗传协调和遗传稳定性。The hybrid progenies from wide cross T.timopheevi×Avena fatua L.var.Portugal were analyzed using SRAP markers to prove their truth and understand their characterizations in the present study.Among 22 primer pairs,20 yielded the polymorphic product between the both parents,and the average polymorphic ratio was 73.81%.Four primer pairs（me4-em1,me3-em5,me4-em3 and me3-em3） produced the specific markers for either parent in the F3 line,indicating that the F3 line contained both parental homologues of some chromosomes,and were true intergeneric hybrid origin.The F3 line lost some parental SRAP markers,and the markers lost from T.timopheevi were far less than those from the wild oat;meanwhile,some novel SRAP markers were generated.These DNA sequence changes might contribute to the rapid evolution of the newly formed allopolyploid and allow the divergent genomes to act in harmony.

关 键 词：提莫菲维小麦 葡萄牙野燕麦 远缘杂交 SRAP 

分 类 号：S512.1[农业科学—作物学]