奶牛性控冻精人工授精效果的研究  

Effect of Artificial Insemination with Frozen-sexed Sperm on the Reproduction of Dairy Cow

作　　者：马毅[1] 陈小强[2] 贾福德[1] 田雨泽[1] 张聚忠 朱芳贤[1] 赵庆斌[1] 汪连海[1] 

机构地区：[1]天津市奶牛发展中心,天津300384 [2]天津农学院,天津300384

出　　处：《华北农学报》2010年第1期113-116,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划(2006BAD04A01-112006BAD04A01-21);天津市自然科学基金项目(08JCYBJC04500);天津市农业科技成果转化与推广项目(0603010)

摘　　要：为研究奶牛性控冻精人工授精效果并合理制定人工授精方案,采用流式细胞仪制备性控冻精,对3 947头健康荷斯坦牛进行人工授精。结果表明：①性控冻精和常规冻精的受胎率、产犊率差异不显著（P〉0.05）,但性控冻精的雌犊率和繁殖率分别达到92.01%和50.03%,对照组仅为51.13%和34.97%,差异极显著（P〈0.01）。②青年牛的情期受胎率达到55.25%,极显著高于经产牛（P〈0.01）;青年牛的产犊率达到98.31%,显著高于经产牛（P〈0.05）;青年牛的繁殖率为51.84%,经产牛为42.12%,存在极显著差异（P〈0.01）。③采用第1次人工授精后10-11 h重复授精1次,每次1支性控冻精的授精方案,其情期受胎率为55.17%,繁殖率达到55.81%,同其他方案相比,差异极显著（P〈0.01）。应用性控冻精进行人工授精可以显著提高母牛的繁殖效率。To evaluate the effect of artificial insemination with frozen-sexed sperm on the reproductive performance of dairy cow,and to further improve insemination strategies,frozen spermwassexed by flow cytometry.Then 3 947 health Holstein cow were artificially inseminated with frozen sperm unsexed or sexed.The results showed：①The pregnancy and calving rates were 48.91% and 97.97% for sexed-sperm AIs,51.74% and 98.05% for the unsexed-sperm AIs（P〉0.05）.The proportion of female calves derived from sexed-sperm AIs was 92.01% compared with 51.13% for control AIs（P〈0.01）,the reproductive rate was 51.13% for sexed-sperm AIs and 34.97% for control AIs（P〈0.01）.② The pregnancy rate was 55.25% for primiparous cow and was obviously higher than that of multiparous cow（P〈0.01）,the calving rate was 98.31% for primiparous cow and was higher than that of multiparous cow（P0.05）,the reprodcutive rates was 51.84% for primiparous cow and 42.12% for multiparous cow,there were significant differences（P〈0.01）.③When a insemination was followed by a second one 10—11 hours later,and a single-doses sperm was used in each time,the pregnancy and reproductive rates were both the highest（P〈0.01）.Our results indicated that AI using sexed sperm under field conditions in commercial dairy herds without oestrus synchronization resulted in a significantly improvement of reprocuctive effect compared with AI using unsexed sperm.

关 键 词：奶牛 性别控制 冷冻精液 人工授精 

分 类 号：S814.3[农业科学—畜牧学]