质粒介导的喹诺酮类耐药基因在鸡源大肠杆菌中的流行  

Prevalence of Plasmid-mediated Quinolone Resistance Genes among 16S rRNA Methylase-producing Escherichia coli Strains Isolated from Diseased Chickens

作　　者：肖方[1] 李新生[1] 张素梅[1] 刘河冰[1] 潘玉善[1] 刘建华[1] 杜向党[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《华北农学报》2010年第1期222-225,共4页Acta Agriculturae Boreali-Sinica

基　　金："十一五"国家科技支撑计划(2006BAK02A03-3);河南省青年骨干教师资助计划;河南农业大学青年科技创新基金(30700321)

摘　　要：为了解河南省质粒介导的喹诺酮类耐药基因(qnrA、qnrB、qnrS、qepA和aac(6′)-Ib-cr)在产16S rRNA甲基化酶鸡源大肠杆菌中的流行与分布,分别设计特异性引物对2005-2008年在河南省病鸡病料中分离保存的158株16SrRNA甲基化酶阳性鸡大肠杆菌进行PCR扩增。结果显示,在158株产16S rRNA甲基化酶鸡源大肠杆菌中,qnrA、qnrB、qnrS、qepA和aac(6′)-Ib-cr的检出率分别为0.6%,2.5%,13.9%,2.5%和58.2%。提示质粒介导的喹诺酮类耐药基因在河南省产16S rRNA甲基化酶鸡源大肠杆菌中普遍存在,且以qnrS和aac(6′)-Ib-cr为主。In order to investigate the prevalence of plasmid-mediated quinolone resistance determinants（qnrA,qnrB,qnrS,qepA and aac（6′）-Ib-cr） among 16S rRNA methylase-producing Escherichia coli strains isolated from diseased chickens in Henan province,158 16S rRNA methylase-producing Escherichia coli strains isolated from diseased chickens during the years 2005—2008 were subjected to PCR for the presence of plasmid-mediated quinolone resistance determinants.The results revealed,of 158 16S rRNA methylase-producing E.coli strains,qnrA,qnrB,qnrS,qepA and aac（6′）-Ib-cr were detected in 0.6%,2.5%,13.9%,2.5% and 58.2%,respectively,suggesting plasmid-mediated quinolone resistance determinants were prevalent among 16S rRNA methylase-producing Escherichia coli strains isolated from diseased chickens in Henan province,with qnrS and aac（6′）-Ib-cr dominant.

关 键 词：大肠杆菌 喹诺酮类 耐药 质粒介导 

分 类 号：S44[农业科学—农业昆虫与害虫防治]