鸡传染性法氏囊病病毒XX08株VP2高变区基因的克隆与序列分析  

Cloning and Analysis of Highly Variable Region VP2 Gene in Infectious bursal disease virus XX08 Strain

作　　者：岳锋[1] 宁红梅[1] 银梅[1] 葛亚明[1] 朱彦彩[1] 周娟娟[2] 贾文科[1] 王文强[3] 黄小东[3] 王选年[1,4] 

机构地区：[1]河南科技学院,动物科学学院,河南新乡453003 [2]双汇集团养殖部饲料厂,河南漯河462000 [3]河南科技大学动物科技学院,河南洛阳471003 [4]新乡学院,河南新乡453003

出　　处：《动物医学进展》2010年第3期35-39,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30871885)

摘　　要：应用反转录(RT-PCR)技术从河南新乡某鸡场分离的鸡传染性法氏囊病病毒XX08株总RNA中克隆出593bp的VP2高变区基因。序列分析表明,XX08毒株VP2高变区氨基酸序列与欧洲超强毒株UK661、日本超强毒株OKYM以及经典弱毒株D78的同源性均为96.8%。遗传进化分析表明,该毒株与超强毒株UK661和OKYM位于同一进化树。XX08毒株与超强毒株具有相似的氨基酸特征,但是222位的A被P替代,提示XX08毒株可能为中等强毒力毒株。The Infectious bursal disease virus（IBDV）XX08 strain was isolated from a chicken farm in Xinxiang city of Henan province.The highly variable region VP2 gene,593 bp,was cloned from the total RNA of the XX08 strain by RT-PCR.Amino acid sequence analysis showed that there was 96.8% homology among the European vvIBDV strain of OKYM,Japanese UK661,classical attenuated strain D78 and the isolated strain of XX08.Compared with the highly variable region of these strain,the amino acid residue A in 222 position of polyprotein of IBDV was substituted with P.The phylogenetic analysis showed that the XX08 strain was in the same lineage with the European and Japanese vvIBDV.These data suggested that the XX08 strain was probably the moderate virulent IBDV.

关 键 词：传染性法氏囊病病毒 VP2 克隆 

分 类 号：S852.659.3[农业科学—基础兽医学] Q785[农业科学—兽医学]