鸡胚成纤维细胞原代培养及应用  

Primary Culture and Applications in Chicken Embryo Fibroblasts

作　　者：朱国坡[1] 周晓丽[2] 郭延锋[1] 李瑞芳[3] 高均伟[1] 陈仕均[1] 王丽荣[1,4] 刘兴友[1,4] 

机构地区：[1]河南科技学院,河南新乡453003 [2]河南农业大学,河南郑州450002 [3]石河子大学,新疆石河子832003 [4]动物疫病和残留物防控河南省高校工程技术研究中心,河南新乡453003

出　　处：《动物医学进展》2010年第3期112-114,共3页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30771601;30972202);河南省杰出人才创新基金项目(0521001800);河南省高校科技创新人才支持计划项目(2009HASTIT010)

摘　　要：原代培养是指在体外模拟体内生理环境,使从体内取出的组织细胞生存、生长和传代,并维持原有组织细胞的结构和功能特性。鸡胚成纤维细胞具有相对容易获得、增殖能力强、适应性强、良好的耐受性、性状比较稳定等特点,使得其被广泛地应用于分子和细胞生物学研究的许多方面。论文综述了近年来有关鸡胚成纤维细胞的培养技术,如鸡胚的取材、细胞的分离、纯化和培养,同时介绍了应用进展和未来研究方向。Primary culture is defined as simulating the in vivo physiological environment to make the cells survival,growth and passage in vitro,and maintain the original structure and function of cells.Chick embryo fibroblasts have characteristics,such as easy obtaining,high proliferation ability and adaptability,good tolerance,relatively stableness,making them widely used in molecular and cytobiologic research in many ways.The article reviewed the current status of chicken embryo fibroblast culture techniques,such as the selection of embryo,cell separation,purification and culture,and also introduced the application and research directions in the future.

关 键 词：鸡胚成纤维细胞 原代培养 分离 纯化 

分 类 号：Q813.11[生物学—生物工程]