柑橘种间体细胞杂种的核质遗传研究  

Inheritance of nuclear and cytoplasmic components of inter-specific somatic hybrids in Citrus

作　　者：徐小勇[1,2] 刘继红[2] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]华中农业大学.作物遗传改良国家重点实验室,武汉430070

出　　处：《果树学报》2010年第2期183-187,共5页Journal of Fruit Science

基　　金：国家自然科学基金(编号:30200189)

摘　　要：为了解柑橘体细胞杂种的核质遗传组成,采用流式细胞仪(FCM)、简单序列重复(SSR)、相关序列扩增多态性(SRAP)以及酶切扩增多态性序列(CAPS)对此前通过对称融合获得的2株种间体细胞杂种进行了分析。结果表明,在丹西红橘+红江橙种间组合中,分析的2株植株中1株为二倍体非对称体细胞杂种,其核DNA具有双亲的部分DNA,并有重组发生,胞质DNA则来源于红江橙(叶肉亲本);而另1株则是异源四倍体体细胞杂种,其核DNA来源于双亲,并有重组发生,胞质DNA则来源于红江橙。FCM,SSR,SRAP and CAPS were employed to characterize the nuclear and cytoplasmic components of two inter-specific somatic hybrid plants obtained by symmetric fusion.The results showed that in Dancy tangerine（Citrus reticulata）＋ sweet orange（C.sinensis Osbeck cv.Hongjiangcheng） inter-specific combination,one of two analyzed plants was diploid asymmetric somatic hybrid,which inherited nuclear DNA from some of both parents and cytoplasmic composition from sweet orange（leaf parent）;the another one was tetraploid heterokaryonic somatic hybrid,which inherited nuclear DNA from both parents and cytoplasmic composition from sweet orange（leaf parent）,and recombination of nuclear DNA occurred in the two plants.

关 键 词：柑橘 体细胞杂种 FCM SSR SRAP CAPS 

分 类 号：S666[农业科学—果树学]