叶绿体atpB-rbcL序列分析在核果类果树系统发育研究中的应用  

Application of atpB-rbcL noncoding spacer in the phylogeny of stone fruit crops

作　　者：王化坤[1,2] 陶建敏[1] 渠慎春[1] 马瑞娟[3] 房经贵[1] 娄晓鸣[4] 章镇[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省太湖常绿果树技术推广中心,江苏苏州215107 [3]江苏省农业科学院,南京210014 [4]苏州农业职业技术学院,江苏苏州215008

出　　处：《果树学报》2010年第2期213-220,共8页Journal of Fruit Science

基　　金：国家自然科学基金(No.30370987);江苏省应用基础研究(No.BJ200018)

摘　　要：为重建核果类果树的系统发育,选择桃、李、梅、杏、樱桃各4～5个主要种或变种,共24个基因型,测定了其叶绿体非编码区atpB-rbcL序列。以桂樱为外种群,用PAUP软件计算数据集的得分,Modeltest筛选最佳模型和参数,Mega计算遗传距离、变异,最大简约法构建系统发育树。结果表明,1)atpB-rbcL在核果类果树各组间分子进化速率不同,差异公布不均衡;2)樱桃较其他核果类果树原始;李、梅、杏亲缘关系较近,梅、杏关系最近。核果类果树是一个单系群,由一个共同的祖先沿着2个方向进化,一枝进化为樱桃,另一枝沿不同的途径产生桃、李、梅、杏等核果类果树。In order to reconstruct phylogeny of main stone fruit trees,the atpB-rbcL noncoding spacers of chloroplast DNA from 24 genotypes of peach（Amygdalus persica L.）,plum（Prunus salicina Lindl.）,apricot（Armeniaca vulgaris Lam.）,mei（Armeniaca mume Sieb.） and cherry（Cerasus avium Moench.）,4-5 genotypes per species,were sequenced and analyzed.Using Prunus zippeliana as outgroup,the data matrix was tested for scores using 56 evolution models with PAUP,and then the scores were used to screen the best model and parameters with Modeltest.The calculation of the genetic distances and vari-ances and the construction of maximum parsimony tree were done with Mega.The result indicated： molecular evolution rates were different among these 5 stone fruit crops and the distribution of genetic variance was unbalanced;cherry was a less evo-lutional species than other four;mei,plum,and apricot were phylogenetically close,among which mei was closest to apri-cot;in the monophyletic group of stone fruit crops,two clades were formed,suggesting that one ancestry plant bi-direction-ally evolved to cherry and the other four stone fruit crops.

关 键 词：核果类果树 系统发育 ATPB-RBCL 分子进化 

分 类 号：S662[农业科学—果树学]