板栗野生居群遗传多样性研究  

SSR analysis on genetic diversity of wild Chinese chestnut populations

作　　者：黄武刚[1] 程丽莉[1] 周志军 刘建立[3] 

机构地区：[1]北京市农林科学院林业果树研究所,北京100093 [2]昌平林业局,北京102200 [3]北京林业大学水土保持与荒漠化教育部重点实验室,北京100083

出　　处：《果树学报》2010年第2期227-232,共6页Journal of Fruit Science

基　　金：科技部科技支撑项目(2008BAD92B03);北京市农林科学院青年科研基金(2007010315);北京市优秀人才培养项目(2009D002005000001);北京市科委项目(ZO909050140902)

摘　　要：使用从板栗中提取的10个微卫星引物对4个中国栗野生居群的69个个体进行扩增,共检测到84个等位基因,每个位点的等位基因数为4～13,每位点的平均等位基因数目为8.4个,平均有效等位基因数(Ne)为4.998,平均期望杂合度(He)为0.777,平均PIC值为0.739,Nei’s多样性指数(h)为0.771,以陕西汉中居群具有最高的遗传多样性。遗传分化系数Gst仅为0.141。UPGMA树状聚类图表明4个野生群体被分为两部分,其中云南、四川、安徽3个居群距离相近位于同一组,此结果与PCA分析结果一致,2种方法均显示居群间的遗传关系与实际地理分布不完全相关。Ten SSR loci isolated from Castanea mollissima Blume were used to evaluate genetic diversity of the wild Chinese chestnut.84 alleles were successfully amplified from 69 accessions of the four wild populations.With an average level of 8.4,the numbers of alleles per locus ranged from 4 to 13.Average values of Na and effective Ne of alleles were 8.4 and 4.998,re-spectively.Average values of He（mean expected heterozygosity）and PIC（polymorphism informative content） were up to 0.777 and 0.739,respectively.However,the highest genetic diversity was found in the Shaanxi population.The low genetic differentiation（Gst = 0.141）showed that the genetic variation mainly occurred within the populations.UPGMA phenogram showed that the populations of Yunnan,Chongqing and Anhui province were divided into one of the two groups,and that principal coordinate analysis also presented the same result.Two methods showed that no significant correlation between the genetic relationships and geographical distance was revealed among the populations.

关 键 词：野生板栗 微卫星 遗传多样性 

分 类 号：S664.2[农业科学—果树学]