百脉根BIO和豌豆突变位点ELE2的比较基因组定位(英文)  

Genetic Analysis and Comparative Mapping of BIO and ELE2 Loci in Lotus japonicus and Pea

作　　者：李信[1] 赫圣博[1] 庄黎丽[1] Sato Shusei TabataSatoshi Ambrose Mike Rameau Catherine 杨军[5] 胡筱荷[5] 罗达[1,5] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240 [2]Kazusa DNA Research Institute,2-6-7 Kazusa-kamatari,Kisarazu,Chiba 292-0818 [3]Department of Crop Genetics,John Innes Centre,Colney,Norwich NR47UH [4]Station de Genetique et d'Amelioration des Plantes,Institut J.P.Bourgin,UR2541NRA [5]中国科学院,上海生命科学研究院植物生理生态研究所植物分子遗传学国家重点实验室,上海200032

出　　处：《基因组学与应用生物学》2010年第1期3-9,共7页Genomics and Applied Biology

基　　金：supported by projects (2008ZX08009-003;2009ZX08009-112B) from the ministry of agriculture of Chinafor transgenic research

摘　　要：豆科两侧对称花的花瓣具有背腹(DV)的分化以及可变的器官内部(IN)非对称性,在大小与形状上显示出不同的发育特征;因而花瓣的发育为克隆决定植物器官的形状与大小的关键基因提供了很好的实验系统。本研究对百脉根中BIO基因进行研究。百脉根bio突变体具有多效性,既影响花器官内部的对称性也影响器官的大小和育性,豌豆ele突变体的表型与bio相似。定位结果表明BIO和ELE2位于豆科基因组的共线性区段,提示BIO和ELE2可能是同源基因突变所致。本研究利用比较基因组定位方法,将BIO和ELE2候选基因锚定在豆科模式植物百脉根和蒺藜苜蓿基因组含有11个同源基因的BAC重叠群上。BIO和ELE2基因的克隆将有助于揭示豆科花瓣形态和大小调控的分子机理,进而为豆科作物遗传改良提供分子理论基础。Petals in zygomorphic flowers possess dorsoventral （DV） differentiation and variable organ internal （IN） asymmetry,which can be clearly defined by their shape and size,and thus provides a good experimental sys-tem to identify the key regulators in the control of organ shape and size. In this study,a novel locus,BIO ORGANS （BIO） was investigated in Lotus japonicus,whose mutants display deficiency of IN asymmetry in flowers,as well as the pleiotropic defects of enlarged organs and infertility which are similar to elephant ear-like leaf （ele） mutants in pea. It was found that BIO and ELE2 were located in the syntenic regions among papilionoid legume genomes,suggesting that they are the homologous loci. Through comparative genomic mapping,the candidate gene for BIO and ELE2 was anchored in the contigs from the genomes of L. japonicus and Medicago truncatula,which contained eleven putative orthologous genes. Cloning the BIO and ELE2 should shed light on the underlying molecular mechanism controlling petal organ shape and size in legume and further provide the molecular basis for genetic improvement on legume crops.

关 键 词：器官的形状和大小 百脉根 BIO基因 ELE2基因 比较基因组定位 

分 类 号：Q943.2[生物学—植物学]