水稻苗期生长对缺磷响应的QTL定位(英文)  

Mapping of QTL for Seedling Growth Response to Phosphorus Deficiency in Rice (Oryza sativa L.)

作　　者：林冬枝[1] 张永娟[1] 张俊芝[1] 罗利军[2] 董彦君[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海市农业生物基因中心,上海201106

出　　处：《基因组学与应用生物学》2010年第1期10-16,共7页Genomics and Applied Biology

基　　金：supported by National Natural Science Foundation of China (No.30971552);Shanghai MunicipalEducation Commission of China (No. 06ZZ21);Shanghai Municipal Science and Technology Commission of China (No.075405117; No.08PJ14085; 9391912300; 09DJ1400505);Leading Academic Discipline Project of Shanghai Municipal Education Commission(No.J50401)

摘　　要：缺磷是抑制全球水稻产量主要因素之一。本研究利用Asomonori(粳型)/IR24(籼型)杂交重组自交株系,对5个水稻苗期性状(相对苗高、相对根长、相对根重、相对苗重以及相对总重)在缺磷条件下的响应QTL进行定位。共检测到20个水稻苗期生长对缺磷响应的QTL位点,分别位于第1(4QTLs)、第4(4QTLs)、第5(2QTLs)、第7、第8(4QTLs)、第9(2QTLs)、第11(2QTLs)和第12号染色体上,其中13个QTLs位于与C3029C、XNpb302、C621B、C621C、R2976和C1263分子标记紧密连锁的6个基因组区域上。另外,每个性状均能从双亲中检测到正负效应QTL位点,这些能解释重组自交系群体中出现超亲和连续分布的现象。本文主要报道了水稻第5、第7和第11染色体上存在水稻苗期生长对缺磷响应的QTL位点。研究表明,该结果及其中检测到的QTLs两侧的连锁分子标记可用于水稻苗期耐低磷性分子育种。Phosphorus （P） deficiency is one of major factors limiting rice yields worldwide. This study was conducted to map quantitative trait loci （QTL） responsible for the stress-induced alterations （stress/control ratios） in five seedling growth traits under P deficiency,viz.,relative seedling height （RSH）,relative root length （RRL）,relative root dry-weight （RRW）,relative stem and leaf shoot dry-weight （RSW） and relative total dry-weight （RTW） of rice seedlings,representing their growth response to P deficiency using recombinant inbred lines （RILs） derived from a cross between a japonica variety Asominori and an indica variety IR24. Totally,twenty QTLs for seedling growth response to P deficiency were detected on chromosomes 1 （four QTL）,4 （four QTL）,5 （two QTL）,7,8 （four QTL）,9 （two QTL）,11 （two QTL） and 12,respectively,of which thirteen QTL were distributed on the six common genomic regions tightly linked to C3029C,XNpb302,C621B,C621C,R2976 and C1263 RFLP-markers. Furthermore,Alleles with positive and negative effects for each trait detected in both parents explained the transgressive segregations and continuous distributions for each trait in the RIL-population. In this paper we mainly reported on QTL for seedling growth response to P deficiency on chromosomes 5,7 and 11 in rice. The investigation showed that the results and their tightly linked RFLP-markers that flank the QTL might be useful in breeding and selection of rice varieties with tolerance to P deficiency.

关 键 词：QTL 缺磷 苗期生长 水稻 

分 类 号：S511[农业科学—作物学]