毛百合根尖染色体Giemsa C-带分析  

Giemsa C-banding Analysis of Root Tip Chromosome in Lilium dahuricum

作　　者：胡凤荣[1] 刘光欣[1] 席梦利[1] 吴祝华[1] 施季森[1] 

机构地区：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京210037

出　　处：《基因组学与应用生物学》2010年第1期87-90,共4页Genomics and Applied Biology

基　　金：国家科技支撑计划项目(2006BAD01A1803);国家林业局948项目(2007-4-06)共同资助

摘　　要：本研究利用Giemsa C-带方法对毛百合(Lilium dahuricum Ker-Gawl)根尖染色体进行了分析。研究结果表明毛百合试管苗的染色体倍性变异丰富,染色体倍性变异包括二倍体(2n=2×=24)、三倍体(2n=3×=36)、四倍体(2n=4×=48)到六倍体(2n=6×=72)。对二倍体毛百合的C-带结果进行分析,其带型公式为:2n=2×=24=2CI++2CI+T+T++6I+2I++2I++2I+T++2I+T++2I+T++2T++2T+。每条染色体上都显示出显著的特征带,而且带纹的深浅差异明显。强带主要集中在长短臂上。因此,GiemsaC-带方法可以将毛百合(L.dahuricum)的每条染色体区分开。In this paper,we analyzed the root tip chromosome of Lilium dahuricum Ker-Gawl. by employing Giem-sa C-banding approach. The results showed that there were abundant chromosomal ploidy variation ploidy variation in tube-cultured plantlet of Lilium dahuricum,and the chromosomal ploidy variation included diploid （2n=2×=24）,triploid （2n=3×=36）,tetraploid （2n=4×=48） and hexaploid （2n=6×=72）. Result of the diploid Lilium dahuricum Ker-Gawl C-banding research were analized. The C-banding karyotype of diploid Lilium dahuricum was 2n=2×=24= 2CI＋＋2CI＋T＋T＋＋6I＋2I＋＋2I＋＋2I＋T＋＋2I＋T＋＋2I＋T＋＋2T＋＋2T＋,and each chromosome had their own unique band pattern dis-tinguishable from each other. Strong bands were mainly distributed on both the long arms and the short arms. Each chromosome of Lilium dahuricum Ker-Gawl can be distinguished by Giemsa C-banding.

关 键 词：毛百合 根尖染色体 GIEMSA C-带分析 

分 类 号：S682.29[农业科学—观赏园艺]