丝胶对2型糖尿病大鼠脊髓前角神经元NGF表达的影响  

EFFECTS OF SERICINE ON NGF EXPRESSION IN ANTERIOR HORN NEURONS OF TYPE 2 DIABETES MELLITUS RATS MODEL SPINAL CORD

作　　者：钟美蓉[1] 付文亮[1] 付秀美[1] 马洪伟[1] 陈志宏[1] 薛景凤[1] 

机构地区：[1]承德医学院,河北承德067000

出　　处：《承德医学院学报》2010年第1期5-8,共4页Journal of Chengde Medical University

摘　　要：目的:研究丝胶对2型糖尿病大鼠脊髓前角神经元神经生长因子(Nerve growth factor,NGF)表达的影响。方法:40只SD大鼠随机分为4组,正常对照组、糖尿病模型组、丝胶治疗组和阳性对照组,每组均为10只。链脲佐菌素(Streptozotocin,STZ)连续腹腔注射制作2型糖尿病大鼠模型。待模型成功建立后,模型组大鼠不作任何处理,丝胶治疗组大鼠给予丝胶灌胃(2.4g/kg/d),阳性对照组大鼠给予二甲双胍(55.33mg/kg/d)灌胃。应用免疫组织化学染色法观察脊髓前角神经元NGF的表达,并采用MiVnt图像分析系统对免疫组化染色阳性结果进行定量分析。结果:糖尿病模型组大鼠脊髓前角神经元NGF的表达明显低于正常对照大鼠(P<0.01);与糖尿病模型组大鼠比较,丝胶治疗组与阳性对照组大鼠脊髓前角神经元NGF的表达明显增高(P<0.01),且丝胶组与阳性对照组比较无明显差别(P>0.05)。结论:丝胶可明显提高2型糖尿病大鼠脊髓前角神经元NGF的表达,发挥对糖尿病神经病变的保护作用。Objective：To study the effects of sericine on nerve growth factor （NGF） expression of anterior horn neurons in spinal cord of diabetic rats.Methods：40 SD rats were randomly divided into 4 groups （n=10）： normal control group,diabetes model group,sericine treatment group and positive control group.The type 2 diabetes model rats were established by continuous intraperitoneal injection of Streptozotocin （STZ）.After successfully establishing the diabetes rats model, The rats in diabetes model group were not given any more treatment, the rats in sericine treatment group were lavaged with sericine （2.4g/kg/d）, the rats in positive control group were lavaged with metformin （55.33mg/kg/d）. SP immunohistochemistry staining was used to observe the NGF expression in spinal cord, and the MiVnt image analysis system to quantitatively analyze the positive results of immunohistochemistry staining.Results：NGF expression in spinal cord anterior horn neurons of the diabetic rats was obviously lower than the normal control rats （P 〈 0.01）. Compared with diabetic model rats, the NGF expression in spinal cord anterior horn neurons of sericine treatment rats and positive control rats increased obviously （P 〈 0.01）; moreover, there had no obvious differences between sericine treatment group and positive control group （P 〉 0.05）.Conclusions：Sericine can protect neuropathy of diabetes by increasing the NGF expression in spinal cord anterior horn neurons.

关 键 词：丝胶 神经生长因子 糖尿病 脊髓 前角神经元 

分 类 号：R587.1[医药卫生—内分泌]