巴马火麻仁油、蛋白粉和木脂素酰胺类提取物对老年小鼠的抗衰老作用研究  

Anti-aging effect of hemp seed oil,protein and lignanamide of bama on old mice

作　　者：蔡霈[1,2] 付珣[1] 邓安刚[1] 詹雪晶[1] 蔡光明[1] 李顺祥[2] 

机构地区：[1]中国人民解放军302医院全军中药研究所,北京100039 [2]湖南中医药大学药学院,长沙410208

出　　处：《中南药学》2010年第3期165-170,共6页Central South Pharmacy

摘　　要：目的研究广西巴马产火麻仁油、火麻仁蛋白粉与火麻仁木脂素酰胺类提取物的抗衰老作用。方法采用自然衰老小鼠,每日不同浓度火麻仁油(2.5、5、10 mL.kg-1.d-1)、火麻仁蛋白粉(1.3、2.6 g.kg-1.d-1)与火麻仁木脂素酰胺类提取物(0.11、0.22 g.kg-1.d-1)灌胃,持续30 d,最后检测老年小鼠血清中总胆固醇(TC)、甘油三脂(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量,脑组织与肝组织中SOD、谷胱甘肽过氧化物酶(GSH-Px)、MDA含量,脑组织总抗氧化能力(T-AOC),肝脏中ATP酶活力以及皮肤中Hyp含量,取少量皮肤制备天狼星红切片。结果火麻仁油、火麻仁蛋白粉与火麻仁木脂素酰胺类提取物均能显著提高老年小鼠体内的SOD与GSH-Px活力,增强其总抗氧化能力,降低MDA含量,调节血脂,增强ATP酶活力,改善能量代谢,增加皮肤中羟脯氨酸的含量,皮肤天狼星红病理切片也显示胶原蛋白含量明显增加。结论火麻仁油、火麻仁蛋白粉与火麻仁木脂素酰胺类提取物均有较强的抗衰老作用,尤其是火麻仁木脂素酰胺类提取物,具有进一步的研究开发价值。Objective To determine the anti-aging effect of hemp seed oil, protein and lignanamide. Methods We used the natural aged mice, fed them with difference doses of hemp seed oil （2. 5, 5, and 10 mL·kg^-1·d^-1）, protein （1.3, 2.6g·kg^-1·d^-1） andlignanamides （0.11, 0.22 g·kg^-1·d^-1） every day for 30 d, and detected the total cholesterol （TC）, triglyeride （TG）, malonaldehyde （MDA） content and superoxide dismutase （SOD） activity in the blood serum of the old mice; the MDA content, SOD, glutathione peroxidase （GSH-Px） activity in the brain and liver tissue, the total antioxidant capcuity （T-AOC） in the brain tissue, the ATP enzyme viability of the liver as well as the Hyp content in the skin. We used a small piece of skin to prepare for the pathological section stained with sirius red. Results Hemp seed oil, protein and lignanamide could obviously improve SOD and GSH-Px viability in the old mice, enhance its anti-oxidation ability, decrease the MI）A content, regulate the blood fat, ameliorate its energy metabolism, and also increase the content of Hyp in the skin. The pathological section stained with sirius red also showed significant increase of collagen protein. Conclusion Hemp seed oil, protein and lignanamide have anti-aging effect. Lignanamide of hemp seed is worth further research and development.

关 键 词：火麻仁油 火麻仁蛋白粉 木脂素酰胺 抗衰老作用 

分 类 号：R961[医药卫生—药理学]