化痰平喘片定性定量研究  

Quality spscification for Huatanpingchuan tablets

作　　者：刘志刚 刘志武 欧伟 

机构地区：[1]岳阳市一人民医院,湖南岳阳414000

出　　处：《中南药学》2010年第3期199-201,共3页Central South Pharmacy

摘　　要：目的建立化痰平喘片的定性定量分析方法。方法采用薄层色谱法对处方中地龙、黄芩进行定性鉴别，采用高效液相色谱法测定处方中暴马子皮中紫丁香苷的含量。色谱柱为phenomenex ODS C18（200mm×4．6mm，5μm）柱，流动相为甲醇水（25：75），检测波长为265rlrn，流速为1．0mL·min^-1，柱温为25℃。结果薄层色谱鉴别方法专属性强，紫丁香苷进样量在0．209－2．09μg与峰面积线性关系良好（r＝0．9999），平均回收率为97．3％，RSD为0．5％（n＝5）。结论本方法准确、简便、专属性好，可用于化痰平喘片的质量控制。Objective To establish a qualitative and quantitative method for Huatanpingchuan tablets. Methods Pheretima and Radix scutellariae in recipes were identified by TLC. The content of syringin in Cortex syrigae was determined by HPLC. The chromatographic column was phenomenex ODS C18 （200 mm×4.6mm,5μm）. The mobile phase consisted of methanol-water （25 ： 75）, the flow rate was 1.0 mL·min^-1, and the detective wavelength was 265 nm. The column temperature was 25℃. Results The TLC identification was highly specific. The amount of syringin had a good linearity within 0. 209-2.09μg. The average recovery of syringin was 97.3% and RSD was 0.5% （n=5） Conclusion The method is reliable, simple, accurate and reproducible, and can be used for the quality control of Huatanpingchuan tablets.

关 键 词：化痰平喘片 紫丁香苷 定性定量 薄层色谱法 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]