细辛脑注射液对槐定碱致痫大鼠Caspase3及热休克蛋白-70表达的影响  

Effects of Asarone Injection on Caspase 3 and Heat Shock Protein-70 in Sophoridine-induced Epileptic Rats

作　　者：甄军丽[1] 王维平[2] 

机构地区：[1]河北医科大学第二医院 [2]河北医科大学第二附属医院

出　　处：《中西医结合心脑血管病杂志》2010年第3期325-327,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：河北省科技攻关基金项目(No.07276101D-21);河北省自然科学基金资助项目(No.C2007000852)

摘　　要：目的通过对槐定碱致痫大鼠脑内Caspase3及热休克蛋白-70(hsp-70)含量的测定,探讨细辛脑注射液抗癫痫作用的机制。方法随机将42只大鼠分为6组,空白组、模型组、卡马西平阳性对照组及细辛脑低剂量组、中剂量组和高剂量组。通过免疫组化学法和Nissle染色法,研究细辛脑注射液对Caspase3和hsp-70含量的影响。结果研究表明与模型组比较,细辛脑注射液中、高剂量组可显著减轻大鼠癫痫发作(P<0.05),可使槐定碱致痫大鼠的脑组织中减少Caspase3释放,降低hsp-70在大鼠脑内的表达。结论细辛脑注射液具有良好的抗癫痫效果,可减轻大鼠癫痫发作时对脑组织的损伤。Objective To explore the anti-epileptic effect and its mechanism of Asarone injection on Caspase 3 and heat shock protein-70（hsp-70）in sophoridine-induced epileptic rats.Methods Forty-two rats were randomly divided into six groups：Blank group,sophoridine-induced epilepsy model group,carbamazepine group,Asarone injection low dosage group,Asarone injection medium dosage group and Asarone injection high dosage group.The content of Caspase 3 and HSP-70 were detected by immunohistochemistry and Nissle staining.Results Compared with model group,the seizure were reduced significantly and the content of Caspase 3 and HSP-70 in brain tissue were decreased in Asarone high dosage group（P 0.05）.Conclusion Asarone injection had anti-epileptic effect,which could play a role in preventing seizure of rats effectively.

关 键 词：细辛脑注射液 槐定碱 CASPASE3 热休克蛋白-70 

分 类 号：R285.5[医药卫生—中药学]